Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03210 and AZOBR_RS03215 are separated by 136 nucleotidesAZOBR_RS03215 and AZOBR_RS03220 are separated by 113 nucleotidesAZOBR_RS03220 and AZOBR_RS03225 are separated by 91 nucleotidesAZOBR_RS03225 and AZOBR_RS03230 are separated by 1260 nucleotides AZOBR_RS03210: AZOBR_RS03210 - hypothetical protein, at 682,141 to 682,755 _RS03210 AZOBR_RS03215: AZOBR_RS03215 - hypothetical protein, at 682,892 to 683,377 _RS03215 AZOBR_RS03220: AZOBR_RS03220 - 3-dehydroquinate synthase, at 683,491 to 684,627 _RS03220 AZOBR_RS03225: AZOBR_RS03225 - shikimate kinase, at 684,719 to 685,207 _RS03225 AZOBR_RS03230: AZOBR_RS03230 - hypothetical protein, at 686,468 to 687,238 _RS03230
Group Condition AZOBR_RS03210 AZOBR_RS03215 AZOBR_RS03220 AZOBR_RS03225 AZOBR_RS03230
stress Paraquat dichloride 0.02 mg/ml -0.1 -4.5 N.D. N.D. N.D.
stress Sisomicin 0.01 mg/ml +0.0 -4.5 N.D. N.D. N.D.
carbon source D-Gluconic Acid (C) -0.3 -3.1 N.D. N.D. N.D.
nitrogen source D-Alanine (N) +0.1 -3.0 N.D. N.D. N.D.
stress D-Cycloserine 0.125 mg/ml -0.3 -2.1 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.6 -1.7 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -1.9 N.D. N.D. N.D.
anaerobic; rich media LB; anaerobic -0.1 -2.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -2.2 N.D. N.D. N.D.
nitrogen source Glucuronamide (N) -0.3 -1.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.6 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.6 -1.4 N.D. N.D. N.D.
nitrogen source L-Phenylalanine (N) -0.4 -1.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -1.7 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -1.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -1.2 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.3 -1.9 N.D. N.D. N.D.
stress Chlorite 1 mM -0.0 -1.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -0.7 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 -1.4 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 -1.8 N.D. N.D. N.D.
stress 3-octanone 6.3476 mM -0.3 +1.3 N.D. N.D. N.D.
stress 2-Furfuraldehyde 0.2 vol% -0.3 +1.3 N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) -0.6 +1.9 N.D. N.D. N.D.
nitrogen source L-Glutamic (N) -0.1 +1.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 +2.1 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay +0.5 +1.1 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.5 +1.2 N.D. N.D. N.D.
stress Benzethonium chloride 0.003 mM +0.2 +1.8 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 +2.6 N.D. N.D. N.D.
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