Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03180 and AZOBR_RS03185 overlap by 4 nucleotidesAZOBR_RS03185 and AZOBR_RS03190 are separated by 33 nucleotidesAZOBR_RS03190 and AZOBR_RS03195 are separated by 73 nucleotidesAZOBR_RS03195 and AZOBR_RS03200 are separated by 165 nucleotides AZOBR_RS03180: AZOBR_RS03180 - hypothetical protein, at 678,481 to 678,876 _RS03180 AZOBR_RS03185: AZOBR_RS03185 - putative transcriptional regulator AbrB family, at 678,873 to 679,139 _RS03185 AZOBR_RS03190: AZOBR_RS03190 - aerobic cobaltochelatase subunit CobS, at 679,173 to 680,177 _RS03190 AZOBR_RS03195: AZOBR_RS03195 - molecular chaperone DnaJ, at 680,251 to 680,835 _RS03195 AZOBR_RS03200: AZOBR_RS03200 - BolA family transcriptional regulator, at 681,001 to 681,273 _RS03200
Group Condition AZOBR_RS03180 AZOBR_RS03185 AZOBR_RS03190 AZOBR_RS03195 AZOBR_RS03200
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 N.D. -6.3 +0.1 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 N.D. -3.8 -0.2 -0.4
nitrogen source Parabanic Acid (N) +0.1 N.D. -4.6 -0.2 +0.4
nitrogen source L-Threonine (N) +0.1 N.D. -4.4 -0.1 +0.5
carbon source a-Ketoglutaric (C) +0.2 N.D. -3.6 -0.2 -0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.0 N.D. -2.9 -0.4 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 N.D. -3.3 -0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 N.D. -0.6 -0.2 -1.5
motility inner cut, LB soft agar motility assay -0.0 N.D. -3.2 +0.2 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.2 N.D. -2.9 +0.0 -0.9
nitrogen source Cytosine (N) +0.3 N.D. -3.6 -0.1 -0.1
nitrogen source nitrate (N) -0.0 N.D. -2.4 +0.1 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.2 N.D. -1.9 -0.3 -0.5
nitrogen source Glucuronamide (N) +0.5 N.D. -3.3 -0.2 +0.3
nitrogen source L-Threonine (N) +0.2 N.D. -2.8 -0.2 +0.2
nitrogen source L-Leucine (N) +0.1 N.D. -2.5 -0.2 +0.1
nitrogen source Urea (N) +0.2 N.D. -2.2 -0.1 -0.3
stress Sisomicin 0.01 mg/ml +0.4 N.D. -2.4 +0.1 -0.5
nitrogen source L-Glutamic (N) +0.2 N.D. -2.1 -0.4 +0.2
carbon source D-Gluconic Acid (C) +0.3 N.D. -2.1 -0.7 +0.3
stress D-Cycloserine 0.125 mg/ml +0.7 N.D. -1.6 -0.7 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -2.2 -0.5 +1.1
nitrogen source nitrate (N) +0.2 N.D. -2.4 +0.0 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 N.D. -2.5 -0.1 +0.4
carbon source D-Gluconic Acid (C) +0.6 N.D. -2.1 -0.8 +0.7
carbon source L-Lactate (C) +0.6 N.D. -2.1 +0.0 +0.2
nitrogen source Cytosine (N) +0.2 N.D. -2.0 -0.1 +0.6
nitrogen source L-Lysine (N) +0.4 N.D. -2.0 -0.1 +0.5
nitrogen source L-Isoleucine (N) +0.5 N.D. -1.8 -0.8 +1.5
stress Nickel (II) chloride 0.5 mM -0.4 N.D. +2.8 -0.1 -0.3
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