Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03130 and AZOBR_RS03135 are separated by 260 nucleotidesAZOBR_RS03135 and AZOBR_RS03140 are separated by 12 nucleotidesAZOBR_RS03140 and AZOBR_RS03145 are separated by 184 nucleotidesAZOBR_RS03145 and AZOBR_RS03150 are separated by 147 nucleotides AZOBR_RS03130: AZOBR_RS03130 - hypothetical protein, at 667,704 to 668,327 _RS03130 AZOBR_RS03135: AZOBR_RS03135 - orotidine 5'-phosphate decarboxylase, at 668,588 to 669,301 _RS03135 AZOBR_RS03140: AZOBR_RS03140 - N-(5'-phosphoribosyl)anthranilate isomerase, at 669,314 to 669,958 _RS03140 AZOBR_RS03145: AZOBR_RS03145 - diguanylate cyclase, at 670,143 to 671,018 _RS03145 AZOBR_RS03150: AZOBR_RS03150 - uracil phosphoribosyltransferase, at 671,166 to 671,816 _RS03150
Group Condition AZOBR_RS03130 AZOBR_RS03135 AZOBR_RS03140 AZOBR_RS03145 AZOBR_RS03150
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.0 -3.0 N.D. -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.3 -0.7 N.D. +0.5 -2.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -2.5 N.D. -1.7 +0.6
nitrogen source D-Alanine (N) -0.5 -2.8 N.D. -0.3 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.2 -3.1 N.D. -0.4 -0.4
nitrogen source casaminos (N) +0.5 -2.7 N.D. -1.4 -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.1 -1.8 N.D. -1.0 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.1 -1.5 N.D. -0.1 -2.0
nitrogen source L-Aspartic Acid (N) -1.1 -1.7 N.D. -0.1 -0.9
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.5 -1.3 N.D. -0.3 -1.5
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.8 -2.3 N.D. -0.8 +0.7
nitrogen source L-Asparagine (N) -0.1 -2.9 N.D. -0.5 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -1.8 N.D. -1.9 +1.1
carbon source D-Lactate (C) -0.2 -3.0 N.D. +0.1 +0.2
stress D-Cycloserine 0.125 mg/ml -0.5 +0.9 N.D. +0.4 -3.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.6 -0.6 N.D. +0.0 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.3 N.D. +0.9 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.0 -1.4 N.D. +0.0 +1.0
nitrogen source L-Threonine (N) +0.2 -2.7 N.D. -0.3 +0.7
carbon source D-Gluconic Acid (C) +0.2 -2.9 N.D. -0.4 +1.2
nitrogen source L-Leucine (N) +0.8 -2.7 N.D. +0.2 +0.0
stress Cobalt chloride 0.3 mM -0.2 +0.5 N.D. +0.6 -2.4
nitrogen source L-Citrulline (N) +0.4 -1.8 N.D. -0.6 +0.9
lb LB -0.0 +0.7 N.D. -3.0 +1.5
nitrogen source Parabanic Acid (N) +0.0 -1.8 N.D. -0.6 +1.5
stress Sisomicin 0.01 mg/ml +0.4 +0.9 N.D. +0.3 -2.0
motility outer cut, LB soft agar motility assay +1.0 -0.1 N.D. +0.8 -2.1
nitrogen source L-Aspartic Acid (N) +0.4 -2.0 N.D. -0.2 +1.7
nitrogen source Ammonium chloride (N) +0.2 -1.6 N.D. -0.2 +1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.8 N.D. +1.2 +1.5
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