Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03110 and AZOBR_RS03115 are separated by 88 nucleotidesAZOBR_RS03115 and AZOBR_RS03120 are separated by 88 nucleotidesAZOBR_RS03120 and AZOBR_RS03125 are separated by 14 nucleotidesAZOBR_RS03125 and AZOBR_RS03130 are separated by 286 nucleotides AZOBR_RS03110: AZOBR_RS03110 - integration host factor subunit beta, at 664,483 to 664,767 _RS03110 AZOBR_RS03115: AZOBR_RS03115 - hypothetical protein, at 664,856 to 665,245 _RS03115 AZOBR_RS03120: AZOBR_RS03120 - ornithine cyclodeaminase, at 665,334 to 666,290 _RS03120 AZOBR_RS03125: AZOBR_RS03125 - FAD dependent oxidoreductase, at 666,305 to 667,417 _RS03125 AZOBR_RS03130: AZOBR_RS03130 - hypothetical protein, at 667,704 to 668,327 _RS03130
Group Condition AZOBR_RS03110 AZOBR_RS03115 AZOBR_RS03120 AZOBR_RS03125 AZOBR_RS03130
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.5 N.D. +0.2 -0.1 -2.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. -0.3 -0.4 -2.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.4 N.D. -0.3 -1.0 -0.2
stress D-Cycloserine 0.125 mg/ml -1.1 N.D. -0.5 -0.7 -0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.2 N.D. -0.4 -0.0 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -2.0 N.D. +0.1 +0.0 -0.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -2.2 N.D. +0.0 -0.0 -0.2
nitrogen source L-Aspartic Acid (N) -0.5 N.D. -0.4 -0.5 -1.1
carbon source Ethanol (C) -1.4 N.D. -0.3 -0.2 -0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.9 N.D. -0.1 -0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. +0.1 -0.1 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.3 -0.2 -1.4
stress Cobalt chloride 0.2 mM -1.0 N.D. +0.0 -0.5 -0.6
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.9 N.D. +0.3 -0.3 -1.1
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -2.1 N.D. +0.1 +0.3 -0.3
anaerobic; rich media LB; anaerobic -1.7 N.D. +0.3 -0.1 -0.3
motility inner cut, LB soft agar motility assay -0.3 N.D. -1.2 -0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. +0.8 +0.3 -2.0
stress Cobalt chloride 0.3 mM -1.1 N.D. +0.4 -0.6 -0.2
stress Sisomicin 0.01 mg/ml -2.1 N.D. +0.5 -0.2 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.8 N.D. +0.1 +0.1 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.2 N.D. +0.5 -0.2 -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.7 N.D. +0.3 +0.2 -0.1
nitrogen source L-Glutamic (N) -1.2 N.D. +0.4 -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. +0.6 +0.4 -2.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 N.D. +0.4 -0.5 +0.5
motility outer cut, LB soft agar motility assay -0.1 N.D. +0.6 -1.1 +1.0
stress 3-octanone 6.3476 mM +1.8 N.D. -0.1 -0.2 -0.3
stress 2-pentyl-furan 2.671 mM +1.7 N.D. -0.5 +0.2 +0.1
stress 3-octanone 6.3476 mM +1.5 N.D. +0.6 +0.1 -0.3
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