Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03100 and AZOBR_RS03105 are separated by 129 nucleotidesAZOBR_RS03105 and AZOBR_RS03110 are separated by 322 nucleotidesAZOBR_RS03110 and AZOBR_RS03115 are separated by 88 nucleotidesAZOBR_RS03115 and AZOBR_RS03120 are separated by 88 nucleotides AZOBR_RS03100: AZOBR_RS03100 - 30S ribosomal protein S1, at 661,551 to 663,260 _RS03100 AZOBR_RS03105: AZOBR_RS03105 - membrane protein, at 663,390 to 664,160 _RS03105 AZOBR_RS03110: AZOBR_RS03110 - integration host factor subunit beta, at 664,483 to 664,767 _RS03110 AZOBR_RS03115: AZOBR_RS03115 - hypothetical protein, at 664,856 to 665,245 _RS03115 AZOBR_RS03120: AZOBR_RS03120 - ornithine cyclodeaminase, at 665,334 to 666,290 _RS03120
Group Condition AZOBR_RS03100 AZOBR_RS03105 AZOBR_RS03110 AZOBR_RS03115 AZOBR_RS03120
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.3 -2.2 N.D. -0.4
motility inner cut, LB soft agar motility assay N.D. -0.7 -0.3 N.D. -1.2
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -0.0 -2.1 N.D. +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.4 -2.2 N.D. +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 -1.9 N.D. +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.3 -1.9 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -1.3 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.0 -1.4 N.D. -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.1 -1.7 N.D. +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 -1.8 N.D. +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.4 -2.0 N.D. +0.1
carbon source succinate (C) N.D. +0.1 -1.2 N.D. -0.4
carbon source D,L-Lactate (C) N.D. +0.2 -1.1 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 -1.5 N.D. +0.2
nitrogen source D-Alanine (N) N.D. +0.2 -1.2 N.D. -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.2 -1.6 N.D. +0.1
nitrogen source L-Glutamic (N) N.D. -0.5 -1.2 N.D. +0.4
carbon source Ethanol (C) N.D. +0.4 -1.4 N.D. -0.3
nitrogen source Parabanic Acid (N) N.D. +0.1 -1.0 N.D. -0.4
anaerobic; rich media LB; anaerobic N.D. +0.1 -1.7 N.D. +0.3
stress Sisomicin 0.01 mg/ml N.D. +0.5 -2.1 N.D. +0.5
stress D-Cycloserine 0.125 mg/ml N.D. +0.5 -1.1 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.9 -0.5 N.D. +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 -1.2 N.D. +0.5
stress Cobalt chloride 0.3 mM N.D. +0.1 -1.1 N.D. +0.4
nitrogen source nitrate (N) N.D. -0.5 -0.6 N.D. +0.7
lb LB N.D. +0.2 +0.8 N.D. +0.6
stress 2-pentyl-furan 2.671 mM N.D. +0.4 +1.7 N.D. -0.5
stress 3-octanone 6.3476 mM N.D. +0.1 +1.8 N.D. -0.1
stress 3-octanone 6.3476 mM N.D. +0.0 +1.5 N.D. +0.6
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