Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS03030 and AZOBR_RS03035 are separated by 217 nucleotidesAZOBR_RS03035 and AZOBR_RS03040 are separated by 527 nucleotidesAZOBR_RS03040 and AZOBR_RS03050 are separated by 3714 nucleotidesAZOBR_RS03050 and AZOBR_RS03055 are separated by 103 nucleotides AZOBR_RS03030: AZOBR_RS03030 - hypothetical protein, at 644,658 to 644,927 _RS03030 AZOBR_RS03035: AZOBR_RS03035 - hypothetical protein, at 645,145 to 646,629 _RS03035 AZOBR_RS03040: AZOBR_RS03040 - hypothetical protein, at 647,157 to 647,375 _RS03040 AZOBR_RS03050: AZOBR_RS03050 - diguanylate cyclase, at 651,090 to 652,010 _RS03050 AZOBR_RS03055: AZOBR_RS03055 - molybdopterin biosynthesis protein, at 652,114 to 653,274 _RS03055
Group Condition AZOBR_RS03030 AZOBR_RS03035 AZOBR_RS03040 AZOBR_RS03050 AZOBR_RS03055
stress 2-pentyl-furan 1.335528 mM N.D. -0.4 N.D. -0.3 -0.4
carbon source D-Gluconic Acid (C) N.D. -0.2 N.D. -0.7 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.7 N.D. -0.3 +0.2
stress Sisomicin 0.01 mg/ml N.D. -0.8 N.D. -0.7 +0.7
stress copper (II) chloride 2 mM N.D. +0.3 N.D. -0.1 -0.7
nitrogen source L-Isoleucine (N) N.D. -0.7 N.D. +0.1 +0.2
motility outer cut, LB soft agar motility assay N.D. -0.7 N.D. -0.4 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.1 N.D. -0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 N.D. +0.1 +0.5
stress Cobalt chloride 0.2 mM N.D. -0.2 N.D. -0.3 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.4 N.D. -0.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 N.D. -0.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.5 N.D. -0.4 +0.3
stress 2-pentyl-furan 1.780666667 mM N.D. +0.5 N.D. -0.3 +0.2
lb LB N.D. -0.3 N.D. -0.3 +1.2
lb LB N.D. -0.3 N.D. +0.1 +0.8
stress 3-octanone 6.3476 mM N.D. +0.8 N.D. +0.1 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.2 N.D. +0.6 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.3 N.D. +0.8 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.4 N.D. -0.4 +0.7
stress Cobalt chloride 0.3 mM N.D. -0.3 N.D. -0.2 +1.4
stress Chlorite 1 mM N.D. +0.6 N.D. -0.3 +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.2 N.D. +1.1 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.1 N.D. +0.9 +0.2
nitrogen source L-Glutamic (N) N.D. +0.6 N.D. +0.3 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.1 N.D. +0.7 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.1 N.D. +0.8 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.3 N.D. +0.8 +0.4
stress D-Cycloserine 0.125 mg/ml N.D. +0.1 N.D. +0.3 +1.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.2 N.D. +1.1 +0.7
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