Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS02005 and AZOBR_RS02010 are separated by 88 nucleotidesAZOBR_RS02010 and AZOBR_RS02015 are separated by 211 nucleotidesAZOBR_RS02015 and AZOBR_RS02020 overlap by 4 nucleotidesAZOBR_RS02020 and AZOBR_RS02025 are separated by 3 nucleotides AZOBR_RS02005: AZOBR_RS02005 - transcriptional regulator, at 411,326 to 411,751 _RS02005 AZOBR_RS02010: AZOBR_RS02010 - glutamine amidotransferase, at 411,840 to 412,601 _RS02010 AZOBR_RS02015: AZOBR_RS02015 - S-adenosylmethionine decarboxylase, at 412,813 to 413,322 _RS02015 AZOBR_RS02020: AZOBR_RS02020 - spermidine synthase, at 413,319 to 414,179 _RS02020 AZOBR_RS02025: AZOBR_RS02025 - transporter, at 414,183 to 415,673 _RS02025
Group Condition AZOBR_RS02005 AZOBR_RS02010 AZOBR_RS02015 AZOBR_RS02020 AZOBR_RS02025
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 N.D. -0.1 -4.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.2 N.D. -0.7 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.2 N.D. -1.4 -0.4
stress Benzethonium chloride 0.003 mM N.D. -0.1 N.D. -0.5 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 N.D. -1.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 N.D. -0.9 -0.3
carbon source D-Gluconic Acid (C) N.D. -0.2 N.D. -0.3 -0.5
stress D-Cycloserine 0.125 mg/ml N.D. -0.1 N.D. +0.0 -0.9
stress Paraquat dichloride 0.04 mg/ml N.D. +0.1 N.D. -0.2 -0.7
nitrogen source L-Histidine (N) N.D. +0.1 N.D. -0.3 -0.6
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 N.D. +0.3 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 N.D. -1.3 +0.7
carbon source succinate (C) N.D. -0.2 N.D. +0.2 -0.7
stress Sisomicin 0.01 mg/ml N.D. +0.2 N.D. -0.7 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 N.D. -1.3 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 N.D. -0.8 -0.2
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 N.D. +0.3 -0.7
carbon source butyrate (C) N.D. -0.0 N.D. +0.2 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 N.D. -1.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 N.D. -1.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 N.D. -0.7 +0.2
stress 2-pentyl-furan 1.780666667 mM N.D. +0.1 N.D. +0.4 -0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.1 N.D. -0.8 +0.5
nitrogen source L-Threonine (N) N.D. -0.3 N.D. -0.3 +0.8
stress 2-pentyl-furan 1.780666667 mM N.D. -0.1 N.D. +0.3 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.1 N.D. -0.0 +1.0
nitrogen source nitrate (N) N.D. +0.3 N.D. +0.3 +0.8
stress 2-Furfuraldehyde 0.2 vol% N.D. +0.4 N.D. -0.2 +1.1
lb LB N.D. +0.3 N.D. +0.2 +1.2
motility inner cut, LB soft agar motility assay N.D. +0.6 N.D. +0.2 +1.2
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