Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01990 and AZOBR_RS01995 are separated by 48 nucleotidesAZOBR_RS01995 and AZOBR_RS02000 are separated by 25 nucleotidesAZOBR_RS02000 and AZOBR_RS02005 are separated by 466 nucleotidesAZOBR_RS02005 and AZOBR_RS02010 are separated by 88 nucleotides AZOBR_RS01990: AZOBR_RS01990 - phosphoglycerate mutase, at 408,723 to 409,307 _RS01990 AZOBR_RS01995: AZOBR_RS01995 - exodeoxyribonuclease III, at 409,356 to 410,150 _RS01995 AZOBR_RS02000: AZOBR_RS02000 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 410,176 to 410,859 _RS02000 AZOBR_RS02005: AZOBR_RS02005 - transcriptional regulator, at 411,326 to 411,751 _RS02005 AZOBR_RS02010: AZOBR_RS02010 - glutamine amidotransferase, at 411,840 to 412,601 _RS02010
Group Condition AZOBR_RS01990 AZOBR_RS01995 AZOBR_RS02000 AZOBR_RS02005 AZOBR_RS02010
stress D-Cycloserine 0.125 mg/ml -1.8 -1.0 -0.3 N.D. -0.1
stress Paraquat dichloride 0.02 mg/ml -2.1 -0.3 -0.2 N.D. +0.2
stress Cobalt chloride 0.3 mM -0.7 -0.8 -0.4 N.D. -0.1
motility inner cut, LB soft agar motility assay -1.9 -0.6 +0.1 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.7 -0.2 -0.1 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 +0.1 -0.2 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 +0.1 -0.5 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 -0.2 -0.3 N.D. -0.2
stress Cobalt chloride 0.2 mM -0.1 -0.9 -0.7 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.2 -0.6 +0.2 N.D. -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.3 +0.2 -0.2 N.D. +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.1 +0.2 -0.3 N.D. +0.1
motility outer cut, LB soft agar motility assay -0.4 -0.7 -0.3 N.D. +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.1 +0.2 -0.1 N.D. +0.2
nitrogen source L-Aspartic Acid (N) -1.3 +0.3 +0.3 N.D. +0.1
carbon source D-Gluconic Acid (C) -1.1 -0.1 +0.5 N.D. +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.1 +0.3 +0.1 N.D. +0.1
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.9 +0.3 +0.1 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 -0.4 -0.0 N.D. -0.4
nitrogen source casaminos (N) -0.5 +0.2 +0.5 N.D. +0.3
nitrogen source D-Alanine (N) -0.5 +0.4 +0.4 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 -0.3 +0.3 N.D. +0.3
stress Chlorite 1 mM +1.3 -0.2 -0.2 N.D. +0.1
nitrogen source L-Threonine (N) +0.7 +0.2 +0.5 N.D. -0.3
lb LB +1.4 -0.7 +0.3 N.D. +0.3
carbon source L-Malic (C) +0.6 +0.2 +0.3 N.D. +0.4
nitrogen source L-Threonine (N) +0.8 +0.1 +0.4 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.6 +0.2 +0.6 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +1.7 -0.1 +0.5 N.D. +0.2
motility inner cut, LB soft agar motility assay +2.5 +0.2 +0.2 N.D. +0.6
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