Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01710 and AZOBR_RS01715 are separated by 242 nucleotidesAZOBR_RS01715 and AZOBR_RS01720 are separated by 217 nucleotidesAZOBR_RS01720 and AZOBR_RS01725 are separated by 140 nucleotidesAZOBR_RS01725 and AZOBR_RS01735 are separated by 3110 nucleotides AZOBR_RS01710: AZOBR_RS01710 - translocase, at 348,824 to 349,498 _RS01710 AZOBR_RS01715: AZOBR_RS01715 - exlusion protein FxsA, at 349,741 to 350,241 _RS01715 AZOBR_RS01720: AZOBR_RS01720 - preprotein translocase subunit SecB, at 350,459 to 350,956 _RS01720 AZOBR_RS01725: AZOBR_RS01725 - transcription termination factor Rho, at 351,097 to 352,353 _RS01725 AZOBR_RS01735: AZOBR_RS01735 - death-on-curing protein, at 355,464 to 355,907 _RS01735
Group Condition AZOBR_RS01710 AZOBR_RS01715 AZOBR_RS01720 AZOBR_RS01725 AZOBR_RS01735
motility outer cut, LB soft agar motility assay N.D. -1.3 -5.4 N.D. -0.3
stress 3-octanone 6.3476 mM N.D. -4.2 -2.2 N.D. -0.0
stress 2-pentyl-furan 1.780666667 mM N.D. -4.5 -0.6 N.D. -0.2
stress 3-octanone 5.2896 mM N.D. -3.3 -1.0 N.D. -0.2
motility inner cut, LB soft agar motility assay N.D. -2.7 -1.3 N.D. +0.2
stress 3-octanone 6.3476 mM N.D. -1.3 -2.7 N.D. +0.3
lb no stress control LB no stress control N.D. -0.7 -2.3 N.D. -0.2
stress 1-octen-3-ol 0.6030326667 mM N.D. -0.6 -2.1 N.D. -0.1
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. -0.7 -1.9 N.D. -0.2
stress 3-octanone 5.2896 mM N.D. -1.2 -0.8 N.D. -0.7
stress 3-octanone 5.2896 mM N.D. -0.8 -2.0 N.D. +0.2
nitrogen source L-Glutamic (N) N.D. -0.1 -2.3 N.D. -0.2
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM N.D. -1.3 -1.4 N.D. +0.1
stress 1-octen-3-ol 0.7753276667 mM N.D. -0.1 -1.9 N.D. -0.5
stress 2-pentyl-furan 1.335528 mM N.D. +0.4 -2.4 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.5 +0.3 N.D. -0.7
lb no stress control LB no stress control N.D. +0.3 -2.0 N.D. -0.2
dmso control DMSO control N.D. +0.6 -2.0 N.D. -0.2
stress 2-pentyl-furan 1.335528 mM N.D. +0.5 -1.9 N.D. +0.1
stress Paraquat dichloride 0.02 mg/ml N.D. -2.2 +1.7 N.D. -0.1
stress Paraquat dichloride 0.04 mg/ml N.D. -2.7 +2.0 N.D. +0.6
stress Benzethonium chloride 0.003 mM N.D. -1.2 +1.4 N.D. -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +1.4 -1.1 N.D. -0.3
lb LB N.D. -0.7 +1.8 N.D. -0.1
stress Sisomicin 0.01 mg/ml N.D. -0.7 +1.5 N.D. +1.0
stress Chlorite 1 mM N.D. -0.8 +1.8 N.D. +0.8
stress Cobalt chloride 0.2 mM N.D. +0.1 +1.8 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 +0.9 N.D. +0.8
stress D-Cycloserine 0.125 mg/ml N.D. +1.2 +1.8 N.D. +0.4
lb LB N.D. +1.1 +1.7 N.D. +0.6
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