Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01315 and AZOBR_RS01320 are separated by 58 nucleotidesAZOBR_RS01320 and AZOBR_RS01325 are separated by 22 nucleotidesAZOBR_RS01325 and AZOBR_RS01330 are separated by 230 nucleotidesAZOBR_RS01330 and AZOBR_RS01335 are separated by 84 nucleotides AZOBR_RS01315: AZOBR_RS01315 - GCN5 family N-acetyltransferase, at 273,136 to 275,820 _RS01315 AZOBR_RS01320: AZOBR_RS01320 - hypothetical protein, at 275,879 to 276,451 _RS01320 AZOBR_RS01325: AZOBR_RS01325 - 2-hydroxyacid dehydrogenase, at 276,474 to 277,463 _RS01325 AZOBR_RS01330: AZOBR_RS01330 - hypothetical protein, at 277,694 to 278,248 _RS01330 AZOBR_RS01335: AZOBR_RS01335 - hypothetical protein, at 278,333 to 278,926 _RS01335
Group Condition AZOBR_RS01315 AZOBR_RS01320 AZOBR_RS01325 AZOBR_RS01330 AZOBR_RS01335
stress Sisomicin 0.01 mg/ml +0.0 -5.3 +0.1 N.D. +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.8 -1.0 -1.7 N.D. +0.2
carbon source D-Gluconic Acid (C) -0.9 -1.4 -0.7 N.D. -1.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.8 -0.3 -1.0 N.D. +0.1
lb LB -0.0 -2.7 -0.6 N.D. +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.5 +0.2 -1.4 N.D. -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.7 -0.3 -1.1 N.D. +0.3
nitrogen source L-Asparagine (N) +0.0 -2.3 +0.0 N.D. -0.3
motility inner cut, LB soft agar motility assay -0.2 -1.5 -0.6 N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.8 +0.1 -1.0 N.D. +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.5 +0.1 -1.1 N.D. +0.3
nitrogen source D-Alanine (N) -0.3 -0.5 -1.3 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.6 -2.2 N.D. +0.4
motility inner cut, LB soft agar motility assay +0.2 -2.6 +0.4 N.D. +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.5 +0.2 -0.6 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.7 -2.0 N.D. +0.4
nitrogen source L-Isoleucine (N) -0.7 +0.5 -1.0 N.D. -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.9 +0.8 -0.7 N.D. +0.1
motility inner cut, LB soft agar motility assay -0.6 -2.1 -0.1 N.D. +1.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.5 +0.2 -0.3 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.7 -2.8 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +1.1 -2.2 N.D. -0.5
nitrogen source L-Threonine (N) +0.1 -1.8 +0.5 N.D. +0.1
stress Paraquat dichloride 0.04 mg/ml +0.2 -1.3 +0.4 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -2.4 +1.1 N.D. +0.1
stress 2-pentyl-furan 2.671 mM +0.3 -1.0 +0.7 N.D. -0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.3 +0.7 -0.8 N.D. +0.5
nitrogen source L-Glutamic (N) +0.2 +1.6 -0.7 N.D. -0.4
stress 3-octanone 6.3476 mM -0.5 +1.0 +0.2 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +1.3 -0.1 N.D. +0.5
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