Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01270 and AZOBR_RS01275 are separated by 48 nucleotidesAZOBR_RS01275 and AZOBR_RS01280 are separated by 537 nucleotidesAZOBR_RS01280 and AZOBR_RS01285 are separated by 211 nucleotidesAZOBR_RS01285 and AZOBR_RS01290 are separated by 7 nucleotides AZOBR_RS01270: AZOBR_RS01270 - 5-hydroxymethyluracil DNA glycosylase, at 261,098 to 261,940 _RS01270 AZOBR_RS01275: AZOBR_RS01275 - hypothetical protein, at 261,989 to 263,533 _RS01275 AZOBR_RS01280: AZOBR_RS01280 - hypothetical protein, at 264,071 to 265,975 _RS01280 AZOBR_RS01285: AZOBR_RS01285 - ubiquinone biosynthesis methyltransferase UbiE, at 266,187 to 266,933 _RS01285 AZOBR_RS01290: AZOBR_RS01290 - 2-octaprenylphenol hydroxylase, at 266,941 to 268,479 _RS01290
Group Condition AZOBR_RS01270 AZOBR_RS01275 AZOBR_RS01280 AZOBR_RS01285 AZOBR_RS01290
motility inner cut, LB soft agar motility assay +0.2 -2.0 -0.7 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.2 -1.8 +0.1 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.0 -1.2 -0.2 N.D. N.D.
lb LB +0.1 -0.3 -1.0 N.D. N.D.
stress 2-Furfuraldehyde 0.2 vol% -0.3 -0.2 -0.8 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -0.9 -0.3 N.D. N.D.
lb LB +0.2 -0.6 -0.5 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 -0.7 -0.4 N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 -0.5 -0.5 N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -0.4 -0.7 +0.6 N.D. N.D.
stress D-Cycloserine 0.125 mg/ml -0.1 -0.8 +1.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.5 -0.6 N.D. N.D.
stress Paraquat dichloride 0.02 mg/ml +0.0 -0.5 +0.7 N.D. N.D.
carbon source L-Glutamine (C) +0.3 +0.5 -0.6 N.D. N.D.
nitrogen source L-Threonine (N) +0.3 +0.7 -0.8 N.D. N.D.
carbon source succinate (C) -0.3 +0.7 +0.4 N.D. N.D.
anaerobic; rich media LB; anaerobic +0.2 -0.1 +1.0 N.D. N.D.
nitrogen source L-Isoleucine (N) -0.1 +0.9 +0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.5 +0.7 N.D. N.D.
carbon source Ethanol (C) -0.0 +0.5 +0.7 N.D. N.D.
nitrogen source nitrate (N) +0.1 +0.4 +0.7 N.D. N.D.
nitrogen source L-Citrulline (N) +0.1 +0.8 +0.4 N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.5 -0.1 +0.9 N.D. N.D.
nitrogen source Cytosine (N) +0.2 +0.6 +0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.1 +1.2 N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 -0.1 +1.2 N.D. N.D.
nitrogen source casaminos (N) -0.0 +1.0 +0.3 N.D. N.D.
carbon source D-Lactate (C) -0.2 +0.9 +0.8 N.D. N.D.
stress Paraquat dichloride 0.04 mg/ml +0.2 +0.3 +1.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.5 +1.3 N.D. N.D.
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