Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01070 and AZOBR_RS01075 overlap by 1 nucleotidesAZOBR_RS01075 and AZOBR_RS01080 are separated by 75 nucleotidesAZOBR_RS01080 and AZOBR_RS01085 are separated by 309 nucleotidesAZOBR_RS01085 and AZOBR_RS01090 are separated by 106 nucleotides AZOBR_RS01070: AZOBR_RS01070 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 218,753 to 219,247 _RS01070 AZOBR_RS01075: AZOBR_RS01075 - Rrf2 family transcriptional regulator, at 219,247 to 219,702 _RS01075 AZOBR_RS01080: AZOBR_RS01080 - cysteine synthase, at 219,778 to 220,737 _RS01080 AZOBR_RS01085: AZOBR_RS01085 - hypothetical protein, at 221,047 to 221,820 _RS01085 AZOBR_RS01090: AZOBR_RS01090 - thiamine biosynthesis protein ThiF, at 221,927 to 222,715 _RS01090
Group Condition AZOBR_RS01070 AZOBR_RS01075 AZOBR_RS01080 AZOBR_RS01085 AZOBR_RS01090
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. N.D. N.D. -3.6 -2.9
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. N.D. N.D. -3.7 -2.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. N.D. N.D. -3.6 -2.6
stress Chlorite 1 mM N.D. N.D. N.D. -0.4 -5.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. N.D. N.D. -3.5 -1.3
nitrogen source nitrate (N) N.D. N.D. N.D. +0.3 -5.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. N.D. -3.3 -1.3
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. N.D. +0.2 -4.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. N.D. N.D. -3.3 -1.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. N.D. -2.9 -1.4
nitrogen source nitrate (N) N.D. N.D. N.D. +0.3 -4.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. N.D. N.D. -1.9 -1.4
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -3.2 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. N.D. N.D. -2.0 -1.0
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. N.D. N.D. -2.3 -0.5
carbon source L-Leucine (C) N.D. N.D. N.D. -0.2 -2.0
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. N.D. -0.4 -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.3 -1.3
lb no stress control LB no stress control N.D. N.D. N.D. +0.0 -1.6
nitrogen source Parabanic Acid (N) N.D. N.D. N.D. +0.1 -1.6
nitrogen source L-Leucine (N) N.D. N.D. N.D. -0.4 -1.2
stress 2-pentyl-furan 1.335528 mM N.D. N.D. N.D. -0.3 -1.2
dmso control DMSO control N.D. N.D. N.D. -0.1 -1.3
stress copper (II) chloride 2 mM N.D. N.D. N.D. +0.2 -1.4
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. N.D. -1.0 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.4 +1.2
stress Sisomicin 0.01 mg/ml N.D. N.D. N.D. -1.1 +1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.1 +1.3
nitrogen source L-Isoleucine (N) N.D. N.D. N.D. +1.2 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.5 +1.8
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