Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS01060 and AZOBR_RS01065 are separated by 281 nucleotidesAZOBR_RS01065 and AZOBR_RS01070 overlap by 4 nucleotidesAZOBR_RS01070 and AZOBR_RS01075 overlap by 1 nucleotidesAZOBR_RS01075 and AZOBR_RS01080 are separated by 75 nucleotides AZOBR_RS01060: AZOBR_RS01060 - ATPase AAA, at 215,782 to 217,218 _RS01060 AZOBR_RS01065: AZOBR_RS01065 - phosphopantothenate synthase, at 217,500 to 218,756 _RS01065 AZOBR_RS01070: AZOBR_RS01070 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 218,753 to 219,247 _RS01070 AZOBR_RS01075: AZOBR_RS01075 - Rrf2 family transcriptional regulator, at 219,247 to 219,702 _RS01075 AZOBR_RS01080: AZOBR_RS01080 - cysteine synthase, at 219,778 to 220,737 _RS01080
Group Condition AZOBR_RS01060 AZOBR_RS01065 AZOBR_RS01070 AZOBR_RS01075 AZOBR_RS01080
motility inner cut, LB soft agar motility assay -0.6 N.D. N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.6 N.D. N.D. N.D. N.D.
stress Sisomicin 0.01 mg/ml -0.6 N.D. N.D. N.D. N.D.
lb LB -0.4 N.D. N.D. N.D. N.D.
stress D-Cycloserine 0.125 mg/ml -0.4 N.D. N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.4 N.D. N.D. N.D. N.D.
stress Cobalt chloride 0.3 mM -0.4 N.D. N.D. N.D. N.D.
stress Chlorite 1 mM -0.3 N.D. N.D. N.D. N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.3 N.D. N.D. N.D. N.D.
carbon source D-Gluconic Acid (C) -0.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. N.D. N.D. N.D.
stress 2-Furfuraldehyde 0.2 vol% -0.3 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. N.D. N.D. N.D.
nitrogen source L-Aspartic Acid (N) +0.3 N.D. N.D. N.D. N.D.
nitrogen source casaminos (N) +0.3 N.D. N.D. N.D. N.D.
stress 1-octen-3-ol 0.6784116667 mM +0.3 N.D. N.D. N.D. N.D.
carbon source L-Proline (C) +0.3 N.D. N.D. N.D. N.D.
carbon source succinate (C) +0.3 N.D. N.D. N.D. N.D.
nitrogen source L-Lysine (N) +0.3 N.D. N.D. N.D. N.D.
carbon source L-Malic (C) +0.4 N.D. N.D. N.D. N.D.
carbon source L-Glutamine (C) +0.4 N.D. N.D. N.D. N.D.
nitrogen source L-Histidine (N) +0.4 N.D. N.D. N.D. N.D.
nitrogen source L-Phenylalanine (N) +0.4 N.D. N.D. N.D. N.D.
stress 2-pentyl-furan 2.671 mM +0.5 N.D. N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay +0.5 N.D. N.D. N.D. N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.5 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.5 N.D. N.D. N.D. N.D.
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