Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS00640 and AZOBR_RS00645 are separated by 20 nucleotidesAZOBR_RS00645 and AZOBR_RS00650 overlap by 4 nucleotidesAZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotidesAZOBR_RS00655 and AZOBR_RS00660 overlap by 1 nucleotides AZOBR_RS00640: AZOBR_RS00640 - pyrophosphorylase, at 128,739 to 129,566 _RS00640 AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576 _RS00645 AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481 _RS00650 AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833 _RS00655 AZOBR_RS00660: AZOBR_RS00660 - hypothetical protein, at 131,833 to 132,117 _RS00660
Group Condition AZOBR_RS00640 AZOBR_RS00645 AZOBR_RS00650 AZOBR_RS00655 AZOBR_RS00660
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -2.7 -2.4 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.5 -3.1 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.0 -2.5 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.0 -2.5 N.D. N.D. N.D.
stress 2-pentyl-furan 2.671 mM -3.4 +0.0 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.2 -3.0 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.6 -1.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -3.1 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.4 -2.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -3.3 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.8 -2.0 N.D. N.D. N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.4 -2.4 N.D. N.D. N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.4 -1.3 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.3 -2.1 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.8 -0.7 N.D. N.D. N.D.
stress Paraquat dichloride 0.02 mg/ml -1.6 -0.8 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 -2.0 N.D. N.D. N.D.
stress Cobalt chloride 0.3 mM -1.4 -0.7 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.1 -3.1 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 -2.1 N.D. N.D. N.D.
stress 3-octanone 6.3476 mM -2.0 +0.1 N.D. N.D. N.D.
anaerobic; rich media LB; anaerobic -0.1 -1.8 N.D. N.D. N.D.
stress Bacitracin 0.25 mg/ml -1.5 +0.5 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -1.5 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -1.2 N.D. N.D. N.D.
nitrogen source casaminos (N) +0.8 -1.1 N.D. N.D. N.D.
stress D-Cycloserine 0.125 mg/ml +1.0 -1.2 N.D. N.D. N.D.
nitrogen source L-Glutamic (N) -0.7 +1.4 N.D. N.D. N.D.
nitrogen source L-Serine (N) +1.7 -0.9 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.9 +0.4 N.D. N.D. N.D.
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