Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS00050 and AZOBR_RS00055 are separated by 86 nucleotidesAZOBR_RS00055 and AZOBR_RS00060 are separated by 18 nucleotidesAZOBR_RS00060 and AZOBR_RS00065 are separated by 3 nucleotidesAZOBR_RS00065 and AZOBR_RS00070 are separated by 38 nucleotides AZOBR_RS00050: AZOBR_RS00050 - transposase, at 79 to 471 _RS00050 AZOBR_RS00055: AZOBR_RS00055 - transposase, at 558 to 836 _RS00055 AZOBR_RS00060: AZOBR_RS00060 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein, at 855 to 1,928 _RS00060 AZOBR_RS00065: AZOBR_RS00065 - glycerol-3-phosphate transporter membrane protein, at 1,932 to 2,762 _RS00065 AZOBR_RS00070: AZOBR_RS00070 - glycerol-3-phosphate transporter permease, at 2,801 to 3,685 _RS00070
Group Condition AZOBR_RS00050 AZOBR_RS00055 AZOBR_RS00060 AZOBR_RS00065 AZOBR_RS00070
nitrogen source L-Isoleucine (N) -4.1 +0.6 +0.4 +0.0 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.6 -0.7 +0.3 -0.3 -0.6
stress 2-pentyl-furan 1.335528 mM -2.1 -0.2 +0.1 -0.4 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -2.5 +0.3 +0.1 -0.1 +0.1
motility outer cut, LB soft agar motility assay -3.2 +0.7 +0.1 +0.0 +0.2
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -2.1 +0.1 +0.0 -0.1 +0.1
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -1.5 -0.2 +0.0 -0.1 -0.0
stress 2-pentyl-furan 2.671 mM -0.7 -0.5 +0.0 -0.2 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.1 +0.0 -0.1 -0.2 -0.4
stress 2-pentyl-furan 1.335528 mM -0.5 -0.8 -0.2 -0.2 +0.2
carbon source Glycerol (C) -0.8 -0.7 +0.4 +0.1 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.3 +0.4 -0.1 +0.3 +0.1
stress Sisomicin 0.01 mg/ml -0.5 +0.5 -0.1 -0.5 +0.5
motility inner cut, LB soft agar motility assay -0.9 +0.3 -0.2 +0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 -0.3 -0.1 +0.1 -0.4
stress 2-pentyl-furan 1.780666667 mM +0.9 -0.1 +0.0 -0.5 +0.3
nitrogen source L-Lysine (N) +0.8 +0.5 +0.0 -0.1 -0.5
stress 2-pentyl-furan 1.780666667 mM +1.4 -0.3 -0.2 +0.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.6 -0.1 +0.1 +0.3 -0.7
stress Benzethonium chloride 0.003 mM +1.2 +0.4 -0.2 -0.1 +0.0
stress benzoic 0.145 mg/ml +1.1 +0.7 -0.2 -0.0 -0.1
lb LB +1.2 +0.3 -0.3 +0.1 +0.2
stress Paraquat dichloride 0.02 mg/ml +1.0 +0.3 +0.1 -0.1 +0.3
stress 2-Furfuraldehyde 0.2 vol% +1.0 +0.8 +0.2 -0.1 -0.2
carbon source D-Fructose (C) +0.9 +0.4 -0.0 +0.2 +0.3
stress Chlorite 1 mM +1.3 +0.7 -0.2 +0.0 -0.1
nitrogen source L-Threonine (N) +0.7 +0.9 +0.0 -0.0 +0.1
stress Aluminum chloride 5 mM +1.6 +0.6 -0.1 +0.2 +0.1
lb LB +1.3 +0.8 +0.1 +0.3 +0.1
motility inner cut, LB soft agar motility assay +2.3 +1.0 -0.1 -0.1 +0.3
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