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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_30415 and AO356_30420 overlap by 4 nucleotides
AO356_30420 and AO356_30425 overlap by 29 nucleotides
AO356_30425 and AO356_30430 are separated by 410 nucleotides
AO356_30430 and AO356_30435 are separated by 119 nucleotides
AO356_30415: AO356_30415 - pyrroloquinoline quinone biosynthesis protein PqqC, at 7,101,362 to 7,102,114
_30415
AO356_30420: AO356_30420 - pyrroloquinoline quinone biosynthesis protein PqqD, at 7,102,111 to 7,102,386
_30420
AO356_30425: AO356_30425 - pyrroloquinoline quinone biosynthesis protein PqqE, at 7,102,358 to 7,103,527
_30425
AO356_30430: AO356_30430 - hypothetical protein, at 7,103,938 to 7,104,333
_30430
AO356_30435: AO356_30435 - multidrug transporter, at 7,104,453 to 7,105,877
_30435
Group
Condition
AO356
_30415
AO356
_30420
AO356
_30425
AO356
_30430
AO356
_30435
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.7
N.D.
-0.5
-1.5
+0.0
motility
outer cut, LB soft agar motility assay
-2.0
N.D.
-0.2
+0.0
+0.1
carbon source
D-Sorbitol (C)
-0.5
N.D.
-0.8
-0.5
-0.2
pH
Minimal media pH8
-1.0
N.D.
+0.0
-0.9
-0.0
stress
Lomefloxacin 0.0005 mM
-0.4
N.D.
-0.9
-0.4
-0.1
motility
outer cut, LB soft agar motility assay
-0.6
N.D.
+0.1
-0.8
-0.3
carbon source
D-Trehalose (C)
-0.4
N.D.
-0.0
-1.1
-0.2
stress
Nickel (II) chloride 0.625 mM
-0.2
N.D.
-0.5
-0.8
-0.2
anaerobic
Nitrate reduction with yeast extract
-0.8
N.D.
-1.1
+0.6
-0.3
stress
copper (II) chloride 2.5 mM
-0.9
N.D.
-0.4
+0.1
-0.3
nitrogen source
Urea (N)
-0.1
N.D.
-0.2
-1.1
-0.1
nitrogen source
L-tyrosine (N)
-0.4
N.D.
-0.8
-0.3
-0.0
carbon source
L-Arginine (C)
-0.4
N.D.
+0.3
-1.0
-0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.6
N.D.
-0.8
+0.1
+0.1
stress
Paraquat dichloride 0.0625 mg/ml
+0.3
N.D.
-0.6
-0.8
-0.1
nitrogen source
Gly-Glu (N)
-0.6
N.D.
+0.1
-0.5
-0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.0
N.D.
+0.2
-1.2
-0.1
carbon source
Ethanol (C)
-0.5
N.D.
+0.2
-0.5
-0.2
pH
Minimal media pH9
-0.8
N.D.
-0.5
+0.4
-0.1
carbon source
Tween 20 (C)
-1.0
N.D.
+0.0
+0.2
-0.2
motility
outer cut, LB soft agar motility assay
+0.6
N.D.
-0.7
-0.5
-0.1
carbon source
Carbon source D-Glucose 10 mM
+0.8
N.D.
-1.1
-0.3
-0.0
carbon source
D-Alanine (C)
-0.6
N.D.
+0.4
-0.4
-0.0
carbon source
Carbon source D,L-Lactate 10 mM
+0.3
N.D.
-0.7
-0.3
+0.1
stress
nitrite 10 mM
+0.0
N.D.
+0.6
-0.7
-0.2
nitrogen source
Cytosine (N)
+0.3
N.D.
+0.3
-0.7
-0.2
stress
Benzalkonium Chloride 0.016 mg/ml
+0.3
N.D.
-0.4
-0.4
+0.3
stress
Sisomicin 0.001 mg/ml
+0.4
N.D.
+0.3
-0.5
-0.2
stress
KB-half, Pseudo5 supernatant
+1.0
N.D.
-0.1
+1.5
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+1.0
N.D.
+0.5
+1.6
-0.1
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