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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_30105 and AO356_30110 are separated by 13 nucleotides
AO356_30110 and AO356_30115 overlap by 4 nucleotides
AO356_30115 and AO356_30120 are separated by 3 nucleotides
AO356_30120 and AO356_30125 are separated by 591 nucleotides
AO356_30105: AO356_30105 - sarcosine oxidase subunit beta, at 7,025,675 to 7,026,916
_30105
AO356_30110: AO356_30110 - sarcosine oxidase subunit delta, at 7,026,930 to 7,027,232
_30110
AO356_30115: AO356_30115 - aminomethyltransferase, at 7,027,229 to 7,030,126
_30115
AO356_30120: AO356_30120 - sarcosine oxidase, at 7,030,130 to 7,030,714
_30120
AO356_30125: AO356_30125 - xylose isomerase, at 7,031,306 to 7,032,142
_30125
Group
Condition
AO356
_30105
AO356
_30110
AO356
_30115
AO356
_30120
AO356
_30125
anaerobic
Nitrate reduction with yeast extract
-0.3
+0.6
-0.5
+0.3
-1.6
stress
Benzalkonium Chloride 0.016 mg/ml
+0.1
-0.5
+0.2
-0.4
-0.9
stress
Chlorite 0.04 mM
+0.0
-0.6
-0.0
-0.2
-0.6
carbon source
L-Lysine (C)
-0.4
-0.4
-0.1
-0.5
+0.2
carbon source
L-Glutamine (C)
-0.0
-0.3
-0.2
+0.1
-0.8
carbon source
Carbon source D-Glucose 10 mM
-0.3
+0.1
+0.0
-0.4
-0.6
carbon source
Carbon source Sucrose 2.5 mM
-0.1
-0.2
+0.0
-0.1
-0.7
nitrogen source
Cytosine (N)
-0.2
-0.1
-0.0
-0.1
-0.6
carbon source
Carbon source D-Mannitol 10 mM
+0.1
-0.0
+0.1
-0.6
-0.6
stress
Rifampicin 0.0024 mg/ml
-0.1
-0.5
-0.2
+0.3
-0.4
carbon source
L-Histidine (C)
-0.1
-0.6
-0.0
+0.1
-0.3
stress
Spectinomycin 0.0125 mg/ml
-0.2
+0.1
-0.0
-0.5
-0.3
motility
outer cut, LB soft agar motility assay
+0.1
-0.8
+0.1
-0.4
+0.1
nitrogen source
L-Isoleucine (N)
-0.3
+0.0
-0.1
+0.2
-0.5
stress
Nickel (II) chloride 0.625 mM
-0.3
+0.3
+0.1
-0.2
-0.6
carbon source
L-Lysine (C)
-0.3
+0.2
+0.1
+0.0
-0.7
carbon source
Carbon source D-Mannitol 5 mM
-0.2
+0.0
+0.1
+0.2
-0.7
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.2
+0.1
+0.0
-0.5
-0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.2
-0.3
-0.1
-0.2
+0.4
carbon source
L-Lactate (C)
-0.4
+0.3
-0.1
-0.1
-0.2
resistance
20 mM putrescine
+0.2
-0.3
-0.0
+0.1
-0.5
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.4
-0.1
+0.4
-0.3
-0.8
stress
Spectinomycin 0.025 mg/ml
-0.0
-0.4
+0.2
+0.3
-0.2
motility
outer cut, LB soft agar motility assay
-0.5
+0.3
+0.0
+0.2
-0.3
motility
outer cut, LB soft agar motility assay
-0.2
+0.0
-0.1
-0.4
+0.5
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.3
-0.3
-0.1
+0.2
-0.3
nitrogen source
nitrite (N)
-0.2
+0.1
-0.2
+0.4
-0.3
lb
LB
+0.3
-0.2
-0.1
+0.4
-0.3
stress
KB-half, Pseudo5 supernatant
+0.5
-0.2
+0.5
-0.4
-0.1
pH
Minimal media pH7
-0.1
+0.6
-0.0
-0.1
+0.4
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