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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_29975 and AO356_29980 are separated by 124 nucleotides
AO356_29980 and AO356_29985 are separated by 71 nucleotides
AO356_29985 and AO356_29990 are separated by 68 nucleotides
AO356_29990 and AO356_29995 are separated by 38 nucleotides
AO356_29975: AO356_29975 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 6,997,473 to 6,998,324
_29975
AO356_29980: AO356_29980 - hypothetical protein, at 6,998,449 to 6,999,030
_29980
AO356_29985: AO356_29985 - alcohol dehydrogenase, at 6,999,102 to 7,000,217
_29985
AO356_29990: AO356_29990 - cupin, at 7,000,286 to 7,000,813
_29990
AO356_29995: AO356_29995 - 3-hydroxybutyryl-CoA dehydrogenase, at 7,000,852 to 7,001,700
_29995
Group
Condition
AO356
_29975
AO356
_29980
AO356
_29985
AO356
_29990
AO356
_29995
anaerobic
Nitrate reduction with yeast extract
-0.1
+0.1
-0.7
-1.8
+0.2
pH
Minimal media pH7
-0.3
-0.4
-0.2
-1.2
-0.3
carbon source
L-Lactate (C)
+0.1
-0.4
+0.4
-2.4
+0.1
motility
outer cut, LB soft agar motility assay
+0.6
-0.2
+0.2
-2.7
+0.1
motility
outer cut, LB soft agar motility assay
+0.0
-0.3
+0.0
-0.7
-0.9
stress
nitrate 200 mM
-0.4
+0.1
+0.2
-1.7
+0.0
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.0
+0.3
-0.4
-1.5
-0.0
stress
Chlorite 0.04 mM
+0.1
-1.0
+0.0
-1.0
+0.3
carbon source
L-Lysine (C)
+0.4
-0.2
+0.2
-1.6
-0.3
carbon source
Inosine (C)
+0.0
-0.5
+0.2
-0.8
+0.0
carbon source
Carbon source D-Glucose 10 mM
+0.1
-1.0
+0.2
+0.1
-0.3
stress
copper (II) chloride 2.5 mM
+0.4
-0.2
+0.4
-1.3
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
-0.2
+0.3
-0.7
-0.3
carbon source
a-Ketoglutaric (C)
-0.2
-0.2
+0.3
-0.6
-0.1
carbon source
Carbon source Sucrose 2.5 mM
-0.5
-0.6
+0.3
-0.2
+0.2
temperature
LB 20C
-0.1
+0.1
-0.3
-0.6
+0.3
pH
Minimal media pH6
-0.2
-0.0
+0.3
-1.1
+0.3
temperature
LB 30C
+0.2
-0.0
+0.0
-1.0
+0.3
carbon source
Carbon source D-Mannitol 2.5 mM
-0.2
-0.5
+0.4
-0.3
+0.2
stress
nitrite 10 mM
-0.1
-0.4
-0.4
+0.5
+0.1
stress
Fusidic 0.1 mg/ml
-0.4
-0.4
+0.2
+0.3
+0.0
carbon source
L-Aspartic Acid (C)
+0.1
-0.3
-0.1
+0.8
-0.2
carbon source
Carbon source Sucrose 10 mM
+0.2
-0.6
+0.5
+0.3
+0.0
motility
outer cut, LB soft agar motility assay
+0.3
-0.7
+0.4
+0.6
-0.1
carbon source
Carbon source D-Mannitol 5 mM
-0.2
-0.4
+0.4
+0.4
+0.3
stress
Nickel (II) chloride 0.625 mM
+0.3
-0.5
+0.2
+0.6
+0.1
stress
Lomefloxacin 0.0005 mM
+0.4
-0.1
+0.4
+0.5
-0.4
carbon source
Carbon source D,L-Lactate 10 mM
-0.1
-0.3
+0.8
+0.3
+0.4
carbon source
Carbon source D-Mannitol 2.5 mM
-0.0
-0.5
+0.1
+1.2
+0.2
stress
Sisomicin 0.001 mg/ml
+0.1
+0.4
+0.4
+1.1
-0.3
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