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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_29210 and AO356_29215 are separated by 184 nucleotides
AO356_29215 and AO356_29220 overlap by 4 nucleotides
AO356_29220 and AO356_29225 are separated by 80 nucleotides
AO356_29225 and AO356_29230 are separated by 194 nucleotides
AO356_29210: AO356_29210 - AAA family ATPase, at 6,792,786 to 6,794,777
_29210
AO356_29215: AO356_29215 - dehydrogenase, at 6,794,962 to 6,795,984
_29215
AO356_29220: AO356_29220 - hydroxypyruvate isomerase, at 6,795,981 to 6,796,772
_29220
AO356_29225: AO356_29225 - rhizopine-binding protein, at 6,796,853 to 6,797,779
_29225
AO356_29230: AO356_29230 - porin, at 6,797,974 to 6,799,236
_29230
Group
Condition
AO356
_29210
AO356
_29215
AO356
_29220
AO356
_29225
AO356
_29230
anaerobic
Nitrate reduction with yeast extract
+0.1
+0.7
-5.1
-0.3
-0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.4
-0.2
-0.9
-1.0
-0.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.1
-1.0
-0.4
-0.1
nitrogen source
nitrite (N)
-0.1
-0.1
-0.6
-0.4
-0.1
stress
Cholin acetate 20 mM
-0.3
+0.1
-1.0
-0.2
-0.0
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.3
+0.2
-1.0
-0.3
+0.1
stress
Chlorite 0.08 mM
-0.2
+0.1
-0.7
-0.6
-0.0
nitrogen source
Urea (N)
-0.1
-0.2
-0.9
-0.0
+0.2
stress
Bacitracin 2 mg/ml
-0.2
-0.0
-1.1
+0.2
+0.2
stress
Nickel (II) chloride 0.625 mM
-0.3
-0.2
+0.2
-0.6
-0.1
carbon source
L-Lysine (C)
+0.2
+0.2
-0.7
-0.2
-0.1
survival
4C survival; 1152 hrs
+0.1
-0.5
+0.1
-0.5
+0.2
stress
acetate 40 mM
+0.1
-0.1
-1.1
+0.2
+0.3
carbon source
Cytidine (C)
-0.1
+0.4
-1.0
+0.1
+0.1
carbon source
D,L-Lactate (C)
-0.2
+0.1
+0.7
-0.6
-0.0
stress
Aluminum chloride 5 mM
+0.1
+0.1
-0.7
+0.3
+0.1
survival
4C survival; 672 hrs
+0.2
-0.2
+0.6
-0.5
+0.0
stress
Cobalt chloride 0.08 mM
-0.2
+0.3
+0.6
-0.3
-0.3
carbon source
L-Lactate (C)
+0.1
+0.1
+0.7
-0.4
-0.2
carbon source
L-Proline (C)
+0.2
+0.0
+0.6
-0.5
-0.1
carbon source
L-Lactate (C)
+0.2
+0.3
+0.5
-0.5
-0.1
carbon source
pyruvate (C)
+0.0
+0.0
+0.8
-0.5
+0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.3
+0.4
+0.3
-0.2
+0.2
stress
Cisplatin 0.0032 mg/ml
+0.2
-0.2
+0.6
-0.4
+0.2
carbon source
pyruvate (C)
+0.2
+0.1
+0.8
-0.1
-0.4
stress
Sisomicin 0.001 mg/ml
+0.1
+0.2
+0.6
-0.5
+0.3
carbon source
a-Ketoglutaric (C)
+0.0
-0.1
+1.2
-0.2
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
+0.2
-0.3
+0.7
+0.5
+0.1
stress
syringaldehyde 5 mM
+0.1
-0.3
+0.7
+0.6
-0.0
stress
copper (II) chloride 2.5 mM
-0.0
-0.3
+1.1
+0.7
+0.2
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