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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_28535 and AO356_28540 are separated by 17 nucleotides
AO356_28540 and AO356_28545 are separated by 78 nucleotides
AO356_28545 and AO356_28550 are separated by 53 nucleotides
AO356_28550 and AO356_28555 are separated by 4 nucleotides
AO356_28535: AO356_28535 - mannitol dehydrogenase, at 6,631,865 to 6,633,322
_28535
AO356_28540: AO356_28540 - D-mannose transporter
(from data)
, at 6,633,340 to 6,634,632
_28540
AO356_28545: AO356_28545 - alcohol dehydrogenase, at 6,634,711 to 6,635,742
_28545
AO356_28550: AO356_28550 - bifunctional D-altronate/D-mannonate dehydratase, at 6,635,796 to 6,637,007
_28550
AO356_28555: AO356_28555 - ketodeoxygluconokinase, at 6,637,012 to 6,638,013
_28555
Group
Condition
AO356
_28535
AO356
_28540
AO356
_28545
AO356
_28550
AO356
_28555
carbon source
D-Mannose (C)
-1.6
-2.0
N.D.
-1.9
-1.0
stress
Lomefloxacin 0.0005 mM
-1.0
-0.2
N.D.
-0.7
+0.2
nitrogen source
L-Phenylalanine (N)
-0.2
-0.4
N.D.
-0.3
-0.3
carbon source
L-Leucine (C)
-0.5
-0.0
N.D.
-0.3
-0.3
carbon source
D-Glucose (C)
+0.2
-0.3
N.D.
-0.4
-0.5
motility
outer cut, LB soft agar motility assay
+0.4
-0.1
N.D.
-0.7
-0.6
stress
Benzalkonium Chloride 0.016 mg/ml
-0.7
+0.0
N.D.
+0.2
-0.5
stress
methylglyoxal 0.016 vol%
+0.1
-0.2
N.D.
-0.3
-0.4
survival
4C survival; 1152 hrs
-0.0
-0.1
N.D.
+0.2
-0.8
anaerobic
Nitrate reduction with yeast extract
-0.1
-0.3
N.D.
+0.2
-0.3
carbon source
acetate (C)
+0.0
-0.0
N.D.
+0.3
-0.8
temperature
LB 20C
-0.2
-0.3
N.D.
+0.2
-0.2
carbon source
Carbon source D-Mannitol 2.5 mM
-0.2
-0.1
N.D.
+0.2
-0.4
stress
Rifampicin 0.0024 mg/ml
-0.6
-0.1
N.D.
+0.2
+0.2
stress
nitrate 200 mM
-0.4
+0.1
N.D.
-0.3
+0.3
motility
outer cut, LB soft agar motility assay
+0.4
-0.4
N.D.
-0.5
+0.2
stress
acetate 40 mM
-0.3
+0.1
N.D.
-0.3
+0.4
motility
outer cut, LB soft agar motility assay
+0.1
+0.2
N.D.
-0.5
+0.1
carbon source
Carbon source D-Mannitol 5 mM
-0.0
+0.1
N.D.
+0.4
-0.6
carbon source
D-Glucose (C)
+0.2
-0.2
N.D.
+0.2
-0.3
stress
Vanillin 2.5 mM
+0.2
-0.3
N.D.
-0.1
+0.2
carbon source
Carbon source D-Mannitol 5 mM
-0.4
-0.0
N.D.
+0.4
+0.3
stress
Cobalt chloride 0.08 mM
+0.3
-0.1
N.D.
-0.3
+0.5
carbon source
Fumarate (C)
+0.4
-0.0
N.D.
+0.3
-0.2
motility
outer cut, LB soft agar motility assay
+0.5
+0.0
N.D.
-0.5
+0.6
carbon source
Carbon source Sucrose 10 mM
-0.1
+0.2
N.D.
+0.5
+0.2
carbon source
Carbon source Sucrose 2.5 mM
+0.2
+0.2
N.D.
+0.5
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.5
-0.2
N.D.
+0.3
+0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.4
+0.0
N.D.
+0.1
+1.1
stress
KB-half, Pseudo5 supernatant
+0.9
-0.2
N.D.
+0.7
+0.0
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