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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_28335 and AO356_28340 are separated by 307 nucleotides
AO356_28340 and AO356_28345 are separated by 504 nucleotides
AO356_28345 and AO356_28350 are separated by 51 nucleotides
AO356_28350 and AO356_28355 are separated by 39 nucleotides
AO356_28335: AO356_28335 - peptidase, at 6,588,578 to 6,590,299
_28335
AO356_28340: AO356_28340 - GABA permease, at 6,590,607 to 6,592,007
_28340
AO356_28345: AO356_28345 - TetR family transcriptional regulator, at 6,592,512 to 6,593,123
_28345
AO356_28350: AO356_28350 - spermidine/putrescine ABC transporter substrate-binding protein, at 6,593,175 to 6,594,278
_28350
AO356_28355: AO356_28355 - hypothetical protein, at 6,594,318 to 6,595,655
_28355
Group
Condition
AO356
_28335
AO356
_28340
AO356
_28345
AO356
_28350
AO356
_28355
carbon source
D-Lactate (C)
-0.4
-0.1
-1.1
-0.1
-0.1
nitrogen source
D-Alanine (N)
-0.3
-0.2
-0.4
-0.3
-0.6
carbon source
Putrescine (C)
-0.1
-0.3
-1.2
-0.1
-0.1
stress
Nickel (II) chloride 0.625 mM
-0.2
-0.1
-0.8
-0.1
-0.4
motility
outer cut, LB soft agar motility assay
-0.6
-0.5
-0.2
-0.2
+0.0
nitrogen source
D-Serine (N)
-0.0
+0.0
-1.2
-0.0
-0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.0
-0.1
-0.8
-0.1
-0.4
resistance
20 mM putrescine
-0.4
+0.3
-0.9
+0.0
-0.3
carbon source
L-Leucine (C)
-0.4
-0.0
-0.6
+0.0
-0.3
carbon source
L-Histidine (C)
-0.4
-0.1
-0.8
+0.3
-0.3
carbon source
Carbon source D-Mannitol 10 mM
-0.4
-0.2
-0.6
+0.1
+0.1
anaerobic
Nitrate reduction with yeast extract
+0.1
-1.1
+0.1
+0.2
-0.4
carbon source
D-Xylose (C)
-0.4
-0.5
-0.3
+0.1
+0.1
carbon source
L-Histidine (C)
+0.0
+0.2
-1.1
+0.1
-0.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.1
-0.1
-0.7
+0.2
-0.2
temperature
LB 10C
+0.3
-0.2
-0.9
+0.1
-0.1
stress
syringaldehyde 5 mM
-0.2
-0.2
+0.2
+0.2
-0.7
stress
Sisomicin 0.001 mg/ml
-0.2
-0.6
+0.6
+0.1
-0.2
stress
copper (II) chloride 2.5 mM
-0.2
+0.1
+0.8
-0.2
-0.3
stress
Aluminum chloride 5 mM
-0.1
-0.1
+0.8
-0.2
-0.2
carbon source
Carbon source D-Mannitol 2.5 mM
+0.2
-0.2
+0.7
-0.2
-0.3
stress
KB-half, Pseudo5 supernatant
+0.7
+0.2
-0.0
-0.5
-0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.5
+0.2
+0.5
+0.2
-0.2
stress
Chloramphenicol 0.005 mg/ml
-0.2
-0.2
+0.8
-0.1
+0.1
stress
Paraquat dichloride 0.0625 mg/ml
-0.1
-0.1
+0.9
-0.1
-0.2
stress
Chlorite 0.08 mM
+0.1
+0.2
+0.8
-0.2
-0.5
stress
Spectinomycin 0.025 mg/ml
+0.3
-0.4
+0.6
-0.1
+0.2
lb
LB
+0.5
+0.1
+0.4
+0.2
-0.3
stress
Cholin acetate 20 mM
-0.1
+0.4
+0.8
-0.1
+0.0
stress
Paraquat dichloride 0.0625 mg/ml
+0.0
+0.3
+1.3
-0.3
-0.2
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