Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_28220 and AO356_28225 are separated by 385 nucleotidesAO356_28225 and AO356_28230 are separated by 34 nucleotidesAO356_28230 and AO356_28235 are separated by 339 nucleotidesAO356_28235 and AO356_28240 are separated by 17 nucleotides AO356_28220: AO356_28220 - non-ribosomal peptide synthetase, at 6,495,382 to 6,521,169 _28220 AO356_28225: AO356_28225 - ATP-binding protein, at 6,521,555 to 6,523,255 _28225 AO356_28230: AO356_28230 - syrP protein, at 6,523,290 to 6,524,351 _28230 AO356_28235: AO356_28235 - thioester reductase, at 6,524,691 to 6,526,538 _28235 AO356_28240: AO356_28240 - chemotaxis protein CheX, at 6,526,556 to 6,527,491 _28240
Group Condition AO356_28220 AO356_28225 AO356_28230 AO356_28235 AO356_28240
motility outer cut, LB soft agar motility assay +0.2 -0.5 N.D. -0.3 -0.6
stress Chlorite 0.08 mM -0.0 -0.2 N.D. -0.5 -0.4
stress Cephalothin 2 mg/ml -0.1 -0.3 N.D. -0.0 -0.6
stress Choline chloride 300 mM +0.0 +0.0 N.D. -0.3 -0.7
carbon source D,L-Lactate (C) +0.0 -0.3 N.D. -0.4 -0.3
survival 4C survival; 672 hrs +0.3 -0.3 N.D. -0.1 -0.9
lb LB +0.0 -0.1 N.D. -0.1 -0.7
carbon source D-Galactose (C) +0.0 -0.5 N.D. -0.1 -0.2
stress Benzalkonium Chloride 0.016 mg/ml +0.1 +0.2 N.D. -0.3 -0.8
carbon source Tween 20 (C) -0.1 +0.1 N.D. -0.1 -0.7
stress perchlorate 75 mM +0.2 -0.0 N.D. -0.1 -0.7
lb LB -0.1 -0.2 N.D. +0.2 -0.5
carbon source L-Arabinose (C) -0.0 -0.4 N.D. -0.4 +0.1
stress nitrite 10 mM +0.0 -0.3 N.D. +0.2 -0.5
stress 1-ethyl-3-methylimidazolium acetate 20 mM -0.0 +0.2 N.D. +0.1 -0.8
stress nitrate 200 mM +0.2 +0.2 N.D. -0.3 -0.4
carbon source Carbon source D-Mannitol 2.5 mM +0.1 +0.3 N.D. -0.1 -0.5
survival 4C survival; 1152 hrs +0.3 +0.3 N.D. -0.4 -0.3
motility outer cut, LB soft agar motility assay +0.2 -0.2 N.D. -0.6 +0.4
stress Cisplatin 0.0032 mg/ml -0.2 +0.1 N.D. -0.3 +0.4
stress Chlorite 0.04 mM +0.2 +0.3 N.D. -0.2 -0.2
phage P5ss-5 phage 10-1 dilution SM calcl2 -0.2 -0.1 N.D. +0.7 -0.1
nitrogen source D-Serine (N) +0.3 +0.3 N.D. -0.0 -0.3
nitrogen source nitrite (N) +0.1 -0.3 N.D. +0.0 +0.5
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 +0.0 -0.3 N.D. +1.3 -0.6
motility outer cut, LB soft agar motility assay -0.0 -0.4 N.D. +0.1 +0.8
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -0.0 +0.1 N.D. +0.1 +0.7
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.1 +0.1 N.D. +0.3 +0.6
carbon source Carbon source D-Mannitol 5 mM +0.0 +0.3 N.D. +0.3 +0.4
motility outer cut, LB soft agar motility assay -0.1 +0.4 N.D. +0.5 +0.2
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