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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_28150 and AO356_28155 are separated by 194 nucleotides
AO356_28155 and AO356_28160 are separated by 388 nucleotides
AO356_28160 and AO356_28165 are separated by 76 nucleotides
AO356_28165 and AO356_28170 are separated by 625 nucleotides
AO356_28150: AO356_28150 - hypothetical protein, at 6,428,710 to 6,430,188
_28150
AO356_28155: AO356_28155 - excinuclease ABC subunit A, at 6,430,383 to 6,433,040
_28155
AO356_28160: AO356_28160 - hypothetical protein, at 6,433,429 to 6,434,157
_28160
AO356_28165: AO356_28165 - threonine transporter RhtB, at 6,434,234 to 6,434,833
_28165
AO356_28170: AO356_28170 - hypothetical protein, at 6,435,459 to 6,436,232
_28170
Group
Condition
AO356
_28150
AO356
_28155
AO356
_28160
AO356
_28165
AO356
_28170
anaerobic
Nitrate reduction with yeast extract
-0.2
+0.1
-0.3
N.D.
-3.3
carbon source
L-Asparagine (C)
+0.0
+0.1
-1.7
N.D.
-0.2
carbon source
Carbon source D,L-Lactate 10 mM
+0.1
+0.1
-1.3
N.D.
-0.6
carbon source
Carbon source D,L-Lactate 10 mM
+0.0
+0.1
-1.3
N.D.
-0.4
carbon source
Carbon source Sucrose 5 mM
+0.2
+0.1
-1.5
N.D.
-0.4
carbon source
Carbon source Sucrose 10 mM
+0.0
+0.1
-1.1
N.D.
-0.5
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.1
-0.1
+0.0
N.D.
-1.4
carbon source
Carbon source D-Mannitol 10 mM
+0.2
-0.0
-0.5
N.D.
-1.1
motility
outer cut, LB soft agar motility assay
+0.2
-0.0
-0.9
N.D.
-0.6
carbon source
Carbon source D-Mannitol 5 mM
+0.2
+0.1
-1.2
N.D.
-0.5
pH
Minimal media pH7
+0.2
-0.1
-0.8
N.D.
-0.5
carbon source
acetate (C)
+0.2
-0.1
-0.6
N.D.
-0.5
carbon source
Carbon source D-Glucose 10 mM
+0.1
+0.2
-0.5
N.D.
-0.7
motility
outer cut, LB soft agar motility assay
-0.2
+0.3
-0.4
N.D.
-0.6
stress
nitrate 200 mM
+0.1
+0.2
-0.8
N.D.
-0.4
stress
syringaldehyde 10 mM
-0.3
+0.0
-0.8
N.D.
+0.4
stress
Lomefloxacin 0.0005 mM
+0.1
-0.1
+0.5
N.D.
-1.2
carbon source
D-Lactate (C)
+0.0
-0.0
+0.7
N.D.
-0.7
stress
sodium fluoride 6.25 mM
-0.2
-0.2
+0.7
N.D.
-0.4
carbon source
D-Xylose (C)
+0.3
-0.1
+0.6
N.D.
-0.7
carbon source
D,L-Lactate (C)
+0.2
-0.1
+0.9
N.D.
-0.4
stress
Cholin acetate 40 mM
+0.5
-0.1
+0.8
N.D.
-0.4
nitrogen source
Cytosine (N)
-0.2
+0.0
+0.7
N.D.
+0.5
nitrogen source
Adenosine (N)
+0.1
-0.2
+0.7
N.D.
+0.5
stress
methylglyoxal 0.016 vol%
-0.2
+0.2
+0.4
N.D.
+0.8
nitrogen source
Urea (N)
-0.1
-0.1
+0.9
N.D.
+0.5
stress
Cisplatin 0.0032 mg/ml
+0.0
-0.0
+1.2
N.D.
+0.2
carbon source
D-Galactose (C)
-0.0
-0.1
+1.5
N.D.
+0.2
stress
Cobalt chloride 0.08 mM
-0.0
-0.1
+1.2
N.D.
+0.5
motility
outer cut, LB soft agar motility assay
+0.2
+0.3
+1.1
N.D.
+0.1
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