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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_27550 and AO356_27555 are separated by 53 nucleotides
AO356_27555 and AO356_27560 overlap by 4 nucleotides
AO356_27560 and AO356_27565 are separated by 219 nucleotides
AO356_27565 and AO356_27570 are separated by 135 nucleotides
AO356_27550: AO356_27550 - 3-oxoacyl-ACP synthase, at 6,283,163 to 6,284,455
_27550
AO356_27555: AO356_27555 - multidrug transporter, at 6,284,509 to 6,287,589
_27555
AO356_27560: AO356_27560 - hypothetical protein, at 6,287,586 to 6,288,695
_27560
AO356_27565: AO356_27565 - RND transporter, at 6,288,915 to 6,290,378
_27565
AO356_27570: AO356_27570 - short-chain dehydrogenase, at 6,290,514 to 6,291,299
_27570
Group
Condition
AO356
_27550
AO356
_27555
AO356
_27560
AO356
_27565
AO356
_27570
stress
Sisomicin 0.001 mg/ml
-1.1
-0.4
-0.7
+0.3
-0.2
anaerobic
Nitrate reduction with yeast extract
-1.4
+0.0
+0.1
-0.2
-0.6
stress
Benzalkonium Chloride 0.016 mg/ml
-1.1
+0.0
-0.2
-0.2
+0.0
temperature
LB 37C
-1.1
-0.1
+0.1
-0.1
+0.0
stress
Chlorite 0.08 mM
-0.3
+0.1
-0.7
+0.0
-0.3
carbon source
L-Alanine (C)
-1.1
-0.1
+0.2
-0.3
+0.1
carbon source
L-Leucine (C)
-0.4
-0.2
-0.4
-0.3
+0.1
survival
4C survival; 360 hrs
-1.1
+0.0
+0.4
-0.3
+0.1
carbon source
L-Lysine (C)
-0.3
-0.1
+0.2
+0.1
-0.8
pH
Minimal media pH6
-0.7
-0.0
-0.2
+0.2
-0.2
lb
LB
-1.5
-0.2
+0.2
+0.3
+0.3
carbon source
L-Arabinose (C)
-0.6
-0.0
+0.1
+0.2
-0.5
motility
outer cut, LB soft agar motility assay
-1.0
+0.2
-0.0
-0.1
+0.2
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.9
-0.0
+0.4
-0.1
-0.0
stress
Cholin acetate 40 mM
-0.9
-0.3
+0.0
+0.3
+0.2
stress
Paraquat dichloride 0.0625 mg/ml
-0.8
+0.1
+0.4
+0.0
-0.3
nitrogen source
L-Leucine (N)
-0.9
+0.1
+0.4
+0.1
-0.2
survival
4C survival; 192 hrs
-0.8
-0.2
+0.6
-0.0
+0.1
stress
Cephalothin 2 mg/ml
-0.8
-0.0
+0.4
-0.1
+0.1
stress
Nalidixic 0.004 mg/ml
-1.0
+0.1
-0.1
+0.4
+0.1
stress
KB-half, Pseudo5 supernatant
+0.5
-0.1
-0.4
-0.2
-0.2
survival
4C survival; 24 hrs
-0.8
+0.1
+0.4
+0.1
-0.0
stress
nitrate 200 mM
-0.7
-0.1
-0.1
+0.6
-0.1
stress
Paraquat dichloride 0.0625 mg/ml
-0.4
+0.1
-0.4
+0.2
+0.4
stress
Chlorite 0.04 mM
-0.1
+0.3
-0.5
+0.4
-0.2
motility
outer cut, LB soft agar motility assay
-1.2
+0.7
-0.5
+0.5
+0.5
stress
perchlorate 75 mM
-0.6
+0.0
+0.2
+0.5
-0.1
stress
copper (II) chloride 2.5 mM
+0.5
-0.0
-0.5
-0.3
+0.4
stress
Lomefloxacin 0.0005 mM
-0.2
-0.0
+0.5
-0.4
+0.4
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+1.0
+0.1
-0.5
+0.2
-0.5
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