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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_27535 and AO356_27540 are separated by 1 nucleotides
AO356_27540 and AO356_27545 are separated by 7 nucleotides
AO356_27545 and AO356_27550 are separated by 231 nucleotides
AO356_27550 and AO356_27555 are separated by 53 nucleotides
AO356_27535: AO356_27535 - channel protein TolC, at 6,278,577 to 6,279,884
_27535
AO356_27540: AO356_27540 - hemolysin secretion protein D, at 6,279,886 to 6,281,208
_27540
AO356_27545: AO356_27545 - peptidase, at 6,281,216 to 6,282,931
_27545
AO356_27550: AO356_27550 - 3-oxoacyl-ACP synthase, at 6,283,163 to 6,284,455
_27550
AO356_27555: AO356_27555 - multidrug transporter, at 6,284,509 to 6,287,589
_27555
Group
Condition
AO356
_27535
AO356
_27540
AO356
_27545
AO356
_27550
AO356
_27555
stress
Sisomicin 0.001 mg/ml
-0.1
-0.6
-0.3
-1.1
-0.4
anaerobic
Nitrate reduction with yeast extract
-0.6
-0.5
+0.3
-1.4
+0.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.0
-0.8
-0.5
-0.6
-0.2
lb
LB
-0.3
-0.2
+0.2
-1.5
-0.2
stress
Paraquat dichloride 0.0625 mg/ml
-0.4
-0.8
-0.3
-0.4
+0.1
motility
outer cut, LB soft agar motility assay
-0.2
-0.9
+0.1
-1.2
+0.7
nitrogen source
L-Isoleucine (N)
-0.0
-0.5
-0.2
-0.8
-0.0
carbon source
L-Histidine (C)
-0.2
-0.5
-0.5
-0.4
+0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.4
-1.0
-1.0
+0.2
stress
Choline chloride 500 mM
-0.6
-0.4
-0.0
-0.4
+0.1
temperature
LB 37C
+0.1
+0.0
-0.3
-1.1
-0.1
stress
Cholin acetate 40 mM
+0.1
-0.5
+0.3
-0.9
-0.3
stress
Paraquat dichloride 0.0625 mg/ml
+0.1
-0.2
-0.4
-0.8
+0.1
stress
copper (II) chloride 2.5 mM
-0.7
-0.2
-0.7
+0.5
-0.0
carbon source
L-Alanine (C)
-0.2
+0.2
+0.1
-1.1
-0.1
stress
Benzalkonium Chloride 0.016 mg/ml
-0.3
+0.2
+0.2
-1.1
+0.0
nitrogen source
Putrescine (N)
-0.4
+0.3
+0.1
-0.9
-0.0
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.4
-0.7
+0.1
-0.3
-0.1
stress
KB-half, Pseudo5 supernatant
-0.5
-0.4
-0.1
+0.5
-0.1
pH
Minimal media pH8
-0.2
+0.3
+0.4
-1.0
-0.0
nitrogen source
D-Serine (N)
-0.8
+0.5
+0.2
-0.2
-0.1
nitrogen source
Urea (N)
-0.2
-0.8
+0.2
+0.1
+0.4
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.4
-0.1
-0.6
-0.0
+0.4
resistance
20 mM putrescine
+0.2
+0.5
-0.6
-0.2
+0.2
carbon source
L-Arginine (C)
+0.3
-0.7
+0.2
+0.2
+0.1
carbon source
Carbon source Sucrose 2.5 mM
-0.4
+0.4
+0.4
-0.3
+0.2
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.4
+0.4
-0.5
-0.3
+0.4
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.5
+0.2
-0.3
+1.0
+0.1
stress
nitrate 200 mM
+0.5
+0.5
+0.2
-0.7
-0.1
stress
Nickel (II) chloride 0.625 mM
+0.2
+0.4
+0.3
+0.5
+0.1
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