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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_27465 and AO356_27470 are separated by 306 nucleotides
AO356_27470 and AO356_27475 are separated by 151 nucleotides
AO356_27475 and AO356_27480 are separated by 296 nucleotides
AO356_27480 and AO356_27485 are separated by 45 nucleotides
AO356_27465: AO356_27465 - ketoglutarate semialdehyde dehydrogenase, at 6,258,633 to 6,260,213
_27465
AO356_27470: AO356_27470 - 3-phenylpropionic acid transporter, at 6,260,520 to 6,261,962
_27470
AO356_27475: AO356_27475 - shikimate dehydrogenase, at 6,262,114 to 6,262,938
_27475
AO356_27480: AO356_27480 - hypothetical protein, at 6,263,235 to 6,264,242
_27480
AO356_27485: AO356_27485 - hypothetical protein, at 6,264,288 to 6,265,094
_27485
Group
Condition
AO356
_27465
AO356
_27470
AO356
_27475
AO356
_27480
AO356
_27485
survival
4C survival; 360 hrs
-0.0
+0.0
-0.2
-0.0
-1.5
survival
4C survival; 1152 hrs
-0.4
-0.1
-0.0
-0.3
-0.8
stress
KB-half, Pseudo5 supernatant
+0.1
-0.1
-0.5
-0.3
-0.6
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.0
-0.1
-0.3
-0.3
-0.5
anaerobic
Nitrate reduction with yeast extract
-0.1
+0.2
+0.3
+0.0
-1.5
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.3
+0.0
-0.0
-0.0
-0.7
carbon source
L-Lysine (C)
-0.4
+0.2
-0.5
+0.3
-0.6
stress
Aluminum chloride 5 mM
-0.0
-0.0
+0.1
-0.2
-0.9
stress
Nickel (II) chloride 0.625 mM
-0.1
+0.2
-0.4
+0.0
-0.7
nitrogen source
L-tyrosine (N)
+0.2
+0.0
-0.4
-0.1
-0.5
stress
1-ethyl-3-methylimidazolium acetate 5 mM
+0.2
-0.0
-0.2
+0.0
-0.7
carbon source
L-Isoleucine (C)
+0.2
-0.0
-0.3
+0.3
-0.8
carbon source
Carbon source D-Mannitol 10 mM
-0.2
+0.1
+0.2
+0.0
-0.6
carbon source
L-Malic (C)
+0.0
+0.1
+0.2
-0.1
-0.6
carbon source
Carbon source Sucrose 2.5 mM
+0.2
+0.0
+0.4
-0.1
-0.9
carbon source
Carbon source Sucrose 10 mM
+0.2
+0.1
+0.4
-0.2
-0.6
carbon source
Carbon source Sucrose 5 mM
+0.1
-0.0
+0.4
-0.2
-0.5
carbon source
Carbon source D,L-Lactate 10 mM
+0.1
-0.0
+0.4
-0.3
-0.2
motility
outer cut, LB soft agar motility assay
+0.4
+0.1
+0.2
+0.3
-1.0
stress
nitrite 10 mM
-0.1
+0.1
-0.4
-0.2
+0.7
carbon source
Carbon source D-Mannitol 5 mM
+0.3
+0.0
+0.3
+0.1
-0.5
stress
Cobalt chloride 0.08 mM
-0.1
+0.0
-0.3
+0.2
+0.5
stress
Lomefloxacin 0.0005 mM
-0.1
+0.1
+0.1
-0.7
+1.0
carbon source
Carbon source D-Glucose 10 mM
+0.3
+0.1
+0.5
-0.0
-0.3
stress
perchlorate 75 mM
-0.3
+0.0
+0.3
+0.1
+0.4
stress
copper (II) chloride 2.5 mM
+0.0
+0.1
+0.4
-0.2
+0.5
carbon source
Carbon source D-Glucose 10 mM
+0.2
-0.0
+0.5
-0.1
+0.4
carbon source
Fumarate (C)
+0.1
+0.0
+0.1
+0.3
+1.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.3
+0.1
+0.1
+0.2
+1.0
carbon source
Fumarate (C)
+0.2
+0.1
+0.2
+0.4
+1.1
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