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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_27345 and AO356_27350 are separated by 106 nucleotides
AO356_27350 and AO356_27355 are separated by 237 nucleotides
AO356_27355 and AO356_27360 are separated by 76 nucleotides
AO356_27360 and AO356_27365 are separated by 19 nucleotides
AO356_27345: AO356_27345 - phenol degradation protein meta, at 6,232,271 to 6,233,137
_27345
AO356_27350: AO356_27350 - globin, at 6,233,244 to 6,233,768
_27350
AO356_27355: AO356_27355 - amidase, at 6,234,006 to 6,235,520
_27355
AO356_27360: AO356_27360 - nitrile hydratase subunit alpha, at 6,235,597 to 6,236,253
_27360
AO356_27365: AO356_27365 - nitrile hydratase subunit beta, at 6,236,273 to 6,236,926
_27365
Group
Condition
AO356
_27345
AO356
_27350
AO356
_27355
AO356
_27360
AO356
_27365
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.1
-0.5
-0.5
-0.8
-0.7
stress
KB-half, Pseudo5 supernatant
+0.1
-0.3
-0.3
-0.4
-1.3
stress
Thallium(I) acetate 0.008 mg/ml
-0.3
-0.2
-0.2
-1.1
+0.0
anaerobic
Nitrate reduction with yeast extract
+0.1
-0.4
-0.6
-1.3
+0.7
carbon source
pyruvate (C)
+0.1
-0.1
-0.2
-0.9
-0.2
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.0
-0.4
+0.1
-0.6
-0.5
carbon source
pyruvate (C)
+0.3
-0.1
-0.1
-1.1
+0.2
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.3
+0.2
+0.1
-0.7
-0.3
stress
Chlorite 0.08 mM
-0.3
-0.3
-0.2
-0.8
+0.8
carbon source
L-Arabinose (C)
-0.2
-0.0
+0.1
-0.8
+0.3
motility
outer cut, LB soft agar motility assay
+0.5
-0.4
+0.1
-1.2
+0.4
stress
Sisomicin 0.001 mg/ml
+0.3
-0.3
-0.4
-0.7
+0.7
carbon source
L-Leucine (C)
+0.1
-0.0
-0.0
+0.7
-1.0
nitrogen source
L-Leucine (N)
-0.5
-0.4
+0.2
+0.2
+0.3
carbon source
Cytidine (C)
-0.4
+0.2
+0.3
-0.5
+0.1
stress
Benzalkonium Chloride 0.008 mg/ml
-0.1
+0.4
-0.1
-0.6
+0.3
stress
nitrite 10 mM
-0.4
+0.6
-0.1
-0.4
+0.4
stress
Lomefloxacin 0.0005 mM
+0.7
-0.6
+0.3
-0.1
+0.0
carbon source
D-Fructose (C)
-0.2
-0.3
+0.1
-0.1
+0.8
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.2
-0.5
+0.3
+0.2
+0.6
carbon source
Carbon source D-Glucose 10 mM
+0.2
+0.0
+0.1
+0.7
-0.5
lb
LB
+0.5
+0.4
-0.3
+0.3
-0.3
carbon source
L-Lactate (C)
-0.1
+0.3
+0.2
-0.4
+0.6
stress
Benzethonium chloride 0.025 mM
+0.1
+0.3
-0.1
-0.5
+0.9
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.2
-0.1
+0.3
+0.6
+0.3
stress
Choline chloride 500 mM
+0.2
+0.5
+0.4
+0.4
-0.5
stress
Vanillin 2.5 mM
+0.4
+0.2
-0.2
+0.4
+0.3
pH
Minimal media pH8
+0.3
+0.2
+0.2
+0.7
-0.3
carbon source
L-Lysine (C)
+0.2
+0.1
-0.3
+0.9
+0.3
motility
outer cut, LB soft agar motility assay
+0.4
+0.5
+0.0
+0.9
+0.1
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