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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_27010 and AO356_27015 are separated by 146 nucleotides
AO356_27015 and AO356_27020 are separated by 14 nucleotides
AO356_27020 and AO356_27025 overlap by 4 nucleotides
AO356_27025 and AO356_27030 are separated by 141 nucleotides
AO356_27010: AO356_27010 - serine protease, at 6,152,806 to 6,155,898
_27010
AO356_27015: AO356_27015 - peptidase, at 6,156,045 to 6,157,394
_27015
AO356_27020: AO356_27020 - hemolysin D, at 6,157,409 to 6,158,743
_27020
AO356_27025: AO356_27025 - peptidase, at 6,158,740 to 6,160,518
_27025
AO356_27030: AO356_27030 - alkaline proteinase inhibitor, at 6,160,660 to 6,161,097
_27030
Group
Condition
AO356
_27010
AO356
_27015
AO356
_27020
AO356
_27025
AO356
_27030
stress
copper (II) chloride 2.5 mM
-0.0
-0.1
-0.9
-0.2
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.1
+0.4
-0.7
-0.7
N.D.
anaerobic
Nitrate reduction with yeast extract
-0.2
-0.7
+0.5
-0.4
N.D.
pH
Minimal media pH8
+0.3
-0.2
-0.8
-0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.1
+0.1
-0.4
-0.4
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.3
+0.1
-0.2
-0.4
N.D.
stress
Nickel (II) chloride 0.625 mM
-0.1
-0.2
-0.1
-0.3
N.D.
carbon source
D-Sorbitol (C)
-0.1
-0.3
-0.3
-0.0
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.1
-0.2
-0.5
+0.1
N.D.
carbon source
pyruvate (C)
-0.1
+0.2
-0.3
-0.2
N.D.
temperature
LB 30C
-0.0
-0.1
-0.5
+0.2
N.D.
carbon source
L-Lysine (C)
+0.1
-0.2
+0.2
-0.4
N.D.
stress
Choline chloride 500 mM
-0.1
-0.0
+0.2
-0.4
N.D.
stress
Sisomicin 0.001 mg/ml
-0.1
-0.2
+0.3
-0.3
N.D.
stress
Cholin acetate 40 mM
+0.1
-0.3
+0.4
-0.5
N.D.
lb
LB
+0.1
+0.2
-0.2
-0.2
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
+0.5
-0.2
-0.4
N.D.
stress
Vanillin 1.25 mM
+0.1
+0.3
-0.1
-0.3
N.D.
stress
Cephalothin 2 mg/ml
-0.0
+0.1
+0.4
-0.3
N.D.
survival
4C survival; 1152 hrs
+0.4
+0.2
-0.2
-0.2
N.D.
stress
1-ethyl-3-methylimidazolium acetate 10 mM
+0.1
-0.1
+0.5
-0.3
N.D.
stress
syringaldehyde 5 mM
+0.0
+0.0
+0.5
-0.3
N.D.
carbon source
L-Lysine (C)
-0.0
-0.2
+0.6
-0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.0
+0.8
-0.2
-0.2
N.D.
nitrogen source
L-Leucine (N)
+0.1
+0.2
+0.3
-0.1
N.D.
survival
4C survival; 360 hrs
+0.4
+0.1
+0.2
-0.2
N.D.
carbon source
Inosine (C)
-0.1
+0.4
+0.2
-0.0
N.D.
stress
Aluminum chloride 5 mM
+0.0
+0.2
+0.6
+0.1
N.D.
stress
KB-half, Pseudo5 supernatant
+0.8
+0.5
+0.4
+0.2
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.8
+0.5
+0.6
+0.2
N.D.
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