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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_26820 and AO356_26825 are separated by 129 nucleotides
AO356_26825 and AO356_26830 overlap by 1 nucleotides
AO356_26830 and AO356_26835 are separated by 503 nucleotides
AO356_26835 and AO356_26840 are separated by 16 nucleotides
AO356_26820: AO356_26820 - hypothetical protein, at 6,107,407 to 6,107,667
_26820
AO356_26825: AO356_26825 - histidine kinase, at 6,107,797 to 6,109,170
_26825
AO356_26830: AO356_26830 - transcriptional regulator, at 6,109,170 to 6,109,874
_26830
AO356_26835: AO356_26835 - type VI secretion protein ImpA, at 6,110,378 to 6,112,603
_26835
AO356_26840: AO356_26840 - hypothetical protein, at 6,112,620 to 6,113,075
_26840
Group
Condition
AO356
_26820
AO356
_26825
AO356
_26830
AO356
_26835
AO356
_26840
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.7
-0.3
-0.2
-0.6
N.D.
lb
LB
-0.5
-0.3
-0.7
-0.1
N.D.
stress
Bacitracin 2 mg/ml
-0.8
-0.1
-0.6
-0.1
N.D.
carbon source
L-Histidine (C)
-0.8
+0.1
-0.6
-0.1
N.D.
motility
outer cut, LB soft agar motility assay
-0.3
-0.5
-0.1
-0.4
N.D.
motility
outer cut, LB soft agar motility assay
-0.5
-0.3
-0.3
-0.2
N.D.
stress
Thallium(I) acetate 0.008 mg/ml
-0.6
-0.1
-0.6
+0.1
N.D.
stress
Nalidixic 0.004 mg/ml
-1.1
-0.3
+0.1
+0.1
N.D.
survival
4C survival; 1152 hrs
-0.7
+0.1
-0.4
-0.2
N.D.
pH
Minimal media pH6
-1.0
+0.2
-0.3
+0.0
N.D.
stress
Lomefloxacin 0.0005 mM
-0.5
-0.4
+0.2
-0.4
N.D.
stress
Benzalkonium Chloride 0.008 mg/ml
-0.2
-0.6
-0.4
+0.1
N.D.
carbon source
D-Trehalose (C)
-0.7
+0.1
-0.4
+0.1
N.D.
carbon source
L-Lysine (C)
-0.4
+0.0
-0.7
+0.1
N.D.
carbon source
L-Glutamine (C)
+0.0
+0.0
-1.1
+0.2
N.D.
stress
perchlorate 75 mM
-0.2
-0.1
-0.7
+0.2
N.D.
stress
Cisplatin 0.0032 mg/ml
-0.6
+0.1
-0.5
+0.1
N.D.
stress
Choline chloride 500 mM
-0.2
-0.4
-0.4
+0.2
N.D.
stress
nitrate 200 mM
+0.3
-0.3
-0.7
+0.0
N.D.
stress
acetate 40 mM
-1.0
+0.1
+0.3
+0.1
N.D.
carbon source
Carbon source D-Mannitol 2.5 mM
+0.5
-0.2
-0.6
+0.0
N.D.
carbon source
D-Glucose (C)
-0.3
+0.4
-0.5
+0.2
N.D.
carbon source
D,L-Lactate (C)
-0.5
+0.3
+0.4
+0.1
N.D.
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.4
-0.0
+0.6
+0.3
N.D.
carbon source
Inosine (C)
+0.8
+0.1
-0.3
-0.1
N.D.
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.4
-0.0
+0.8
+0.3
N.D.
motility
outer cut, LB soft agar motility assay
+0.5
-0.7
+0.7
+0.3
N.D.
carbon source
casaminos (C)
+0.7
+0.1
+0.3
-0.1
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
-0.0
+1.0
+0.2
N.D.
stress
copper (II) chloride 2.5 mM
+0.7
+0.5
+0.6
+0.3
N.D.
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