Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_26780 and AO356_26785 are separated by 464 nucleotidesAO356_26785 and AO356_26790 are separated by 96 nucleotidesAO356_26790 and AO356_26795 overlap by 11 nucleotidesAO356_26795 and AO356_26800 are separated by 126 nucleotides AO356_26780: AO356_26780 - D-amino acid oxidase, at 6,098,345 to 6,099,670 _26780 AO356_26785: AO356_26785 - LysR family transcriptional regulator, at 6,100,135 to 6,101,019 _26785 AO356_26790: AO356_26790 - alanine racemase, at 6,101,116 to 6,102,252 _26790 AO356_26795: AO356_26795 - reactive intermediate/imine deaminase, at 6,102,242 to 6,102,646 _26795 AO356_26800: AO356_26800 - aldehyde dehydrogenase, at 6,102,773 to 6,104,260 _26800
Group Condition AO356_26780 AO356_26785 AO356_26790 AO356_26795 AO356_26800
stress Nickel (II) chloride 0.625 mM -0.0 N.D. -0.2 -0.7 -0.5
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.0 N.D. -0.7 -0.7 +0.1
stress Sisomicin 0.001 mg/ml +0.5 N.D. -0.5 -0.2 -1.0
nitrogen source D-Glucosamine Hydrochloride (N) -0.3 N.D. -0.3 -0.2 -0.4
stress nitrate 200 mM +0.1 N.D. -0.5 -0.3 -0.3
anaerobic Nitrate reduction with yeast extract -0.4 N.D. +0.0 -0.8 +0.3
nitrogen source Ammonium chloride (N) -0.4 N.D. +0.2 -0.3 -0.2
survival 4C survival; 360 hrs +0.7 N.D. -0.1 -0.5 -0.3
stress nitrite 10 mM +0.4 N.D. +0.2 -0.5 +0.2
stress Paraquat dichloride 0.0625 mg/ml +0.4 N.D. -0.0 +0.6 -0.5
stress Rifampicin 0.0024 mg/ml -0.2 N.D. -0.3 +0.4 +0.5
motility outer cut, LB soft agar motility assay +0.7 N.D. -0.5 +0.5 -0.1
carbon source L-Lysine (C) +0.3 N.D. +0.4 +0.3 -0.4
stress Doxycycline hyclate 0.0001 mg/ml +0.6 N.D. -0.4 +0.5 -0.0
stress Benzalkonium Chloride 0.016 mg/ml +0.3 N.D. -0.8 +0.2 +1.0
carbon source Carbon source D,L-Lactate 10 mM -0.3 N.D. -0.1 +0.2 +0.8
stress methylglyoxal 0.016 vol% +0.4 N.D. +0.1 -0.2 +0.5
carbon source Carbon source D-Glucose 10 mM -0.4 N.D. +0.2 +0.5 +0.5
stress Chlorite 0.08 mM -0.4 N.D. +0.2 +0.6 +0.4
stress KB-half, Pseudo5 supernatant +0.7 N.D. +0.4 -0.5 +0.1
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.5 N.D. +0.2 -0.3 +0.5
carbon source L-Histidine (C) -0.2 N.D. +0.4 +0.4 +0.3
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.2 N.D. +0.3 -0.1 +0.7
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 -0.2 N.D. +0.6 +0.1 +0.6
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.4 N.D. +0.5 -0.2 +0.5
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.4 N.D. +0.2 -0.2 +0.9
carbon source Tween 20 (C) +0.2 N.D. +0.2 +0.4 +0.5
carbon source L-Arginine (C) +0.4 N.D. +0.4 +0.2 +0.3
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.3 N.D. +0.2 +0.3 +0.6
carbon source Carbon source D-Glucose 10 mM -0.2 N.D. +0.3 +0.5 +0.7
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