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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_26655 and AO356_26660 are separated by 2 nucleotides
AO356_26660 and AO356_26665 overlap by 4 nucleotides
AO356_26665 and AO356_26670 are separated by 163 nucleotides
AO356_26670 and AO356_26675 are separated by 446 nucleotides
AO356_26655: AO356_26655 - (2Fe-2S)-binding protein, at 6,067,645 to 6,068,145
_26655
AO356_26660: AO356_26660 - carbon monoxide dehydrogenase, at 6,068,148 to 6,068,954
_26660
AO356_26665: AO356_26665 - carbon monoxide dehydrogenase, at 6,068,951 to 6,071,950
_26665
AO356_26670: AO356_26670 - histidine kinase, at 6,072,114 to 6,074,324
_26670
AO356_26675: AO356_26675 - hypothetical protein, at 6,074,771 to 6,075,313
_26675
Group
Condition
AO356
_26655
AO356
_26660
AO356
_26665
AO356
_26670
AO356
_26675
carbon source
L-Lysine (C)
-1.0
-0.9
+0.1
-0.2
-0.1
stress
Sisomicin 0.001 mg/ml
-0.8
-0.3
-0.2
-0.1
-0.6
stress
Cholin acetate 40 mM
-1.3
-0.3
-0.2
-0.3
+0.1
stress
acetate 40 mM
-2.0
-0.3
+0.1
-0.0
+0.3
pH
Minimal media pH8
-0.8
-0.9
-0.0
-0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.7
-0.9
-0.5
+0.4
+0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.9
+0.3
-0.3
-0.3
-0.1
carbon source
L-Leucine (C)
-0.7
-0.1
-0.6
-0.0
+0.2
carbon source
D-Glucosamine Hydrochloride (C)
-0.7
-0.5
-0.3
+0.1
+0.2
carbon source
L-Malic (C)
-1.2
-0.1
+0.2
+0.2
-0.3
motility
outer cut, LB soft agar motility assay
-1.5
+0.9
+0.1
-0.3
-0.3
carbon source
L-Asparagine (C)
-1.2
+0.4
-0.2
+0.0
-0.1
carbon source
acetate (C)
-0.9
-0.4
-0.1
+0.2
+0.3
motility
outer cut, LB soft agar motility assay
-0.9
+0.1
+0.3
-0.2
-0.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.8
+0.4
+0.1
+0.0
-0.5
anaerobic
Nitrate reduction with yeast extract
-1.7
+0.9
+0.2
-0.3
+0.2
survival
4C survival; 24 hrs
+0.3
-1.1
-0.1
+0.0
+0.2
stress
Chloramphenicol 0.005 mg/ml
-1.0
+0.1
+0.3
-0.2
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
+0.6
-1.0
-0.1
+0.3
-0.0
stress
Paraquat dichloride 0.0625 mg/ml
+0.6
-0.5
+0.2
-0.4
-0.0
stress
Cisplatin 0.0032 mg/ml
+0.7
-0.9
-0.0
+0.2
-0.0
motility
outer cut, LB soft agar motility assay
-0.8
+0.8
+0.1
+0.1
-0.2
lb
LB
-0.7
+0.7
+0.0
-0.1
+0.2
carbon source
Carbon source Sucrose 5 mM
-0.6
+0.5
+0.3
+0.1
-0.1
stress
copper (II) chloride 2.5 mM
+0.7
-0.0
+0.3
-0.6
-0.1
carbon source
acetate (C)
+0.8
+0.4
+0.1
+0.1
+0.2
carbon source
L-Arabinose (C)
+0.8
+0.3
+0.1
+0.1
+0.4
stress
Nickel (II) chloride 0.625 mM
+0.4
+0.8
+0.1
+0.4
+0.2
stress
KB-half, Pseudo5 supernatant
+1.0
+1.6
+0.4
-0.2
-0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.8
+2.2
+0.3
+0.1
-0.2
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