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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_26325 and AO356_26330 are separated by 1 nucleotides
AO356_26330 and AO356_26335 are separated by 67 nucleotides
AO356_26335 and AO356_26340 are separated by 300 nucleotides
AO356_26340 and AO356_26345 are separated by 16 nucleotides
AO356_26325: AO356_26325 - dolichol-phosphate mannosyltransferase, at 5,962,963 to 5,963,706
_26325
AO356_26330: AO356_26330 - Lipid A biosynthesis, N-terminal, at 5,963,708 to 5,964,001
_26330
AO356_26335: AO356_26335 - dolichyl-phosphate-mannose-protein mannosyltransferase, at 5,964,069 to 5,965,526
_26335
AO356_26340: AO356_26340 - AMP-binding protein, at 5,965,827 to 5,967,491
_26340
AO356_26345: AO356_26345 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 5,967,508 to 5,968,269
_26345
Group
Condition
AO356
_26325
AO356
_26330
AO356
_26335
AO356
_26340
AO356
_26345
carbon source
L-Isoleucine (C)
-0.1
-0.5
+0.0
-4.0
-5.9
stress
nitrite 10 mM
-0.4
-0.9
-0.2
-0.2
-0.2
nitrogen source
L-Isoleucine (N)
+0.1
+0.1
+0.0
-0.5
-1.2
carbon source
Carbon source Sucrose 10 mM
-0.4
-0.2
-0.1
-0.3
-0.3
carbon source
Carbon source Sucrose 10 mM
+0.1
-0.5
+0.1
-0.3
-0.6
motility
outer cut, LB soft agar motility assay
-0.2
-0.1
+0.0
-0.3
-0.7
survival
4C survival; 1152 hrs
+0.2
-0.2
-0.1
-0.2
-0.7
temperature
LB 30C
+0.2
-0.2
-0.2
-0.1
-0.7
carbon source
L-Histidine (C)
-0.5
+0.3
-0.1
-0.1
-0.4
motility
outer cut, LB soft agar motility assay
-0.2
+0.0
-0.1
+0.4
-0.6
stress
Benzalkonium Chloride 0.016 mg/ml
-0.2
-0.7
+0.1
+0.1
+0.3
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.7
-0.3
+0.3
+0.1
+0.4
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
+0.1
-0.4
-0.2
-0.3
+0.6
carbon source
D-Xylose (C)
+0.5
-0.3
+0.2
-0.0
-0.4
stress
syringaldehyde 5 mM
+0.0
+0.6
-0.1
-0.1
-0.6
carbon source
L-Aspartic Acid (C)
-0.5
+0.6
-0.1
-0.1
+0.0
carbon source
Carbon source D,L-Lactate 10 mM
-0.1
+0.7
-0.2
-0.1
-0.3
stress
Choline chloride 500 mM
+0.5
+0.2
-0.2
-0.1
-0.4
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
-0.4
-0.1
-0.4
+0.8
motility
outer cut, LB soft agar motility assay
+0.3
-0.7
+0.3
-0.0
+0.3
motility
outer cut, LB soft agar motility assay
+0.3
+0.6
-0.1
-0.2
-0.5
resistance
20 mM putrescine
+0.4
-0.4
-0.2
-0.2
+0.6
carbon source
L-Arginine (C)
+0.1
+0.5
-0.0
+0.1
-0.4
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.4
-0.2
-0.1
-0.1
+0.4
stress
Ceftazidime 0.2 mg/ml
+0.7
-0.6
+0.1
+0.4
-0.2
stress
Cholin acetate 40 mM
+0.7
+0.0
+0.2
-0.1
+0.3
stress
nitrate 200 mM
+0.8
-0.2
-0.1
+0.1
+0.4
carbon source
D-Xylose (C)
+0.4
+0.5
+0.3
-0.0
+0.2
stress
KB-half, Pseudo5 supernatant
+1.2
+0.3
-0.2
-0.1
+0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+1.4
+1.2
-0.2
-0.3
-0.2
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