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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_26115 and AO356_26120 overlap by 4 nucleotides
AO356_26120 and AO356_26125 are separated by 144 nucleotides
AO356_26125 and AO356_26130 are separated by 69 nucleotides
AO356_26130 and AO356_26135 are separated by 236 nucleotides
AO356_26115: AO356_26115 - (2Fe-2S)-binding protein, at 5,912,214 to 5,913,587
_26115
AO356_26120: AO356_26120 - cytochrome C2, at 5,913,584 to 5,913,934
_26120
AO356_26125: AO356_26125 - ABC transporter substrate-binding protein, at 5,914,079 to 5,914,861
_26125
AO356_26130: AO356_26130 - hypothetical protein, at 5,914,931 to 5,915,356
_26130
AO356_26135: AO356_26135 - AraC family transcriptional regulator, at 5,915,593 to 5,916,429
_26135
Group
Condition
AO356
_26115
AO356
_26120
AO356
_26125
AO356
_26130
AO356
_26135
stress
Lomefloxacin 0.0005 mM
-0.2
N.D.
-0.0
N.D.
-1.0
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.2
N.D.
-0.3
N.D.
-0.6
carbon source
L-Asparagine (C)
-0.4
N.D.
-0.1
N.D.
-0.5
carbon source
L-Glutamic (C)
-0.3
N.D.
-0.5
N.D.
-0.2
lb
LB
-0.3
N.D.
-0.5
N.D.
-0.1
carbon source
L-Glutamine (C)
-0.2
N.D.
-0.4
N.D.
-0.2
stress
Cisplatin 0.0032 mg/ml
-0.3
N.D.
-0.2
N.D.
-0.3
carbon source
L-Lysine (C)
-0.4
N.D.
-0.3
N.D.
-0.1
carbon source
L-Arginine (C)
-0.3
N.D.
-0.3
N.D.
-0.2
carbon source
L-Asparagine (C)
-0.2
N.D.
-0.3
N.D.
-0.3
carbon source
D-Trehalose (C)
-0.3
N.D.
-0.2
N.D.
-0.1
stress
syringaldehyde 5 mM
-0.4
N.D.
-0.2
N.D.
-0.1
carbon source
D-Sorbitol (C)
-0.3
N.D.
-0.4
N.D.
+0.1
carbon source
L-Proline (C)
+0.1
N.D.
-0.4
N.D.
-0.3
carbon source
L-Arginine (C)
-0.5
N.D.
-0.2
N.D.
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.4
N.D.
+0.2
N.D.
-0.3
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.1
N.D.
+0.2
N.D.
-0.6
pH
Minimal media pH7
+0.1
N.D.
-0.4
N.D.
-0.2
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.3
N.D.
-0.4
N.D.
+0.3
carbon source
D-Xylose (C)
+0.3
N.D.
-0.5
N.D.
-0.2
nitrogen source
L-Isoleucine (N)
+0.1
N.D.
+0.1
N.D.
-0.5
resistance
20 mM putrescine
+0.1
N.D.
+0.2
N.D.
-0.5
carbon source
D,L-Lactate (C)
+0.3
N.D.
-0.3
N.D.
-0.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.1
N.D.
+0.4
N.D.
-0.4
stress
KB-half, Pseudo5 supernatant
-0.3
N.D.
+0.2
N.D.
+0.3
stress
Aluminum chloride 5 mM
-0.2
N.D.
+0.2
N.D.
+0.3
nitrogen source
Ammonium chloride (N)
+0.3
N.D.
+0.2
N.D.
+0.3
motility
outer cut, LB soft agar motility assay
+0.7
N.D.
+0.2
N.D.
+0.1
carbon source
L-Leucine (C)
+0.1
N.D.
-0.0
N.D.
+2.0
nitrogen source
L-Leucine (N)
-0.3
N.D.
-0.0
N.D.
+5.9
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