Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_25560 and AO356_25565 are separated by 14 nucleotidesAO356_25565 and AO356_25570 are separated by 12 nucleotidesAO356_25570 and AO356_25575 are separated by 52 nucleotidesAO356_25575 and AO356_25580 are separated by 84 nucleotides AO356_25560: AO356_25560 - 2-keto-3-deoxy-L-rhamnonate aldolase, at 5,778,390 to 5,779,187 _25560 AO356_25565: AO356_25565 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 5,779,202 to 5,780,005 _25565 AO356_25570: AO356_25570 - MFS transporter, at 5,780,018 to 5,781,322 _25570 AO356_25575: AO356_25575 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 5,781,375 to 5,781,767 _25575 AO356_25580: AO356_25580 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 5,781,852 to 5,782,709 _25580
Group Condition AO356_25560 AO356_25565 AO356_25570 AO356_25575 AO356_25580
stress copper (II) chloride 2.5 mM -0.1 -0.8 N.D. N.D. -0.9
stress Paraquat dichloride 0.0625 mg/ml -0.2 -0.5 N.D. N.D. -1.0
stress Thallium(I) acetate 0.008 mg/ml -0.6 -0.1 N.D. N.D. -0.6
stress Cisplatin 0.0032 mg/ml -0.0 -0.7 N.D. N.D. -0.4
carbon source D-Fructose (C) +0.2 -0.7 N.D. N.D. -0.6
pH Minimal media pH7 +0.4 -0.5 N.D. N.D. -0.8
nitrogen source Urea (N) +0.0 +0.5 N.D. N.D. -1.2
carbon source Carbon source Sucrose 5 mM -0.0 -1.1 N.D. N.D. +0.4
stress Rifampicin 0.0016 mg/ml -0.6 -0.6 N.D. N.D. +0.5
stress Cobalt chloride 0.08 mM -0.3 +0.4 N.D. N.D. -0.8
anaerobic Nitrate reduction with yeast extract -1.9 +1.6 N.D. N.D. -0.2
stress Chlorite 0.08 mM +0.2 -0.9 N.D. N.D. +0.3
survival 4C survival; 24 hrs +0.1 +0.3 N.D. N.D. -0.8
carbon source Carbon source D-Mannitol 2.5 mM +0.3 -0.9 N.D. N.D. +0.2
carbon source Cytidine (C) -0.3 +0.7 N.D. N.D. -0.7
stress Lomefloxacin 0.0005 mM +0.5 +0.4 N.D. N.D. -1.1
stress Nalidixic 0.004 mg/ml -0.3 -0.5 N.D. N.D. +0.7
nitrogen source Cytosine (N) +0.3 +0.5 N.D. N.D. -0.5
stress Ceftazidime 0.2 mg/ml +0.6 -0.6 N.D. N.D. +0.5
stress nitrate 200 mM -0.4 +0.1 N.D. N.D. +0.7
motility outer cut, LB soft agar motility assay +1.0 -0.6 N.D. N.D. +0.4
motility outer cut, LB soft agar motility assay -0.3 +1.0 N.D. N.D. +0.0
motility outer cut, LB soft agar motility assay +0.1 +1.7 N.D. N.D. -0.9
carbon source Carbon source D-Mannitol 10 mM -0.1 +0.8 N.D. N.D. +0.4
stress 1-ethyl-3-methylimidazolium acetate 10 mM +0.7 +0.1 N.D. N.D. +0.4
stress KB-half, Pseudo5 supernatant +1.3 +0.1 N.D. N.D. -0.1
motility outer cut, LB soft agar motility assay +0.1 +0.5 N.D. N.D. +0.9
stress Cholin acetate 20 mM +0.8 +0.7 N.D. N.D. +0.0
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 +0.8 +0.7 N.D. N.D. +0.1
stress 1-ethyl-3-methylimidazolium chloride 200 mM +0.5 +1.1 N.D. N.D. +0.8
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