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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_25310 and AO356_25315 are separated by 5 nucleotides
AO356_25315 and AO356_25320 overlap by 29 nucleotides
AO356_25320 and AO356_25325 overlap by 4 nucleotides
AO356_25325 and AO356_25330 are separated by 3 nucleotides
AO356_25310: AO356_25310 - hypothetical protein, at 5,727,638 to 5,728,126
_25310
AO356_25315: AO356_25315 - cyclopropane-fatty-acyl-phospholipid synthase, at 5,728,132 to 5,729,394
_25315
AO356_25320: AO356_25320 - hypothetical protein, at 5,729,366 to 5,730,181
_25320
AO356_25325: AO356_25325 - FAD-dependent oxidoreductase, at 5,730,178 to 5,731,425
_25325
AO356_25330: AO356_25330 - short-chain dehydrogenase, at 5,731,429 to 5,732,184
_25330
Group
Condition
AO356
_25310
AO356
_25315
AO356
_25320
AO356
_25325
AO356
_25330
anaerobic
Nitrate reduction with yeast extract
-4.0
+0.1
-0.0
+0.0
+0.2
motility
outer cut, LB soft agar motility assay
-1.5
-0.1
+0.5
+0.4
-0.7
nitrogen source
Gly-Glu (N)
-0.4
+0.1
+0.0
-0.2
-0.7
stress
Spectinomycin 0.025 mg/ml
-0.9
+0.0
-0.2
-0.1
+0.0
stress
acetate 40 mM
-1.1
-0.0
-0.2
-0.1
+0.5
motility
outer cut, LB soft agar motility assay
-0.6
+0.3
+0.1
-0.1
-0.6
pH
Minimal media pH7
-0.7
+0.1
-0.1
+0.2
-0.4
motility
outer cut, LB soft agar motility assay
-0.8
+0.2
-0.2
-0.3
+0.3
temperature
LB 20C
-1.0
+0.2
+0.1
-0.0
-0.1
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.7
+0.2
-0.3
+0.0
-0.0
stress
Bacitracin 2 mg/ml
-0.8
-0.1
-0.0
-0.2
+0.3
stress
Nickel (II) chloride 0.625 mM
-0.8
+0.1
-0.0
+0.2
-0.3
stress
Cholin acetate 40 mM
-0.5
-0.2
-0.2
-0.1
+0.3
carbon source
D-Xylose (C)
-0.8
-0.0
+0.0
+0.1
+0.2
stress
copper (II) chloride 2.5 mM
-1.0
-0.1
+0.3
+0.4
-0.1
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.4
-0.0
+0.2
-0.3
-0.7
temperature
LB 10C
-0.2
+0.1
+0.2
+0.2
-0.7
nitrogen source
Urea (N)
-0.7
+0.1
+0.3
+0.2
+0.0
carbon source
L-Histidine (C)
-0.3
-0.2
+0.1
-0.2
+0.5
carbon source
L-Asparagine (C)
+0.5
+0.1
-0.4
-0.2
+0.1
stress
Chlorite 0.04 mM
+0.1
+0.0
+0.1
-0.5
+0.4
carbon source
Carbon source D,L-Lactate 10 mM
+0.5
-0.2
+0.2
-0.2
-0.2
carbon source
L-Aspartic Acid (C)
+0.8
-0.0
-0.3
-0.0
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.6
-0.6
+0.6
+0.4
+0.5
carbon source
L-Arginine (C)
+0.8
-0.2
-0.1
-0.0
+0.0
carbon source
L-Glutamic (C)
+0.6
-0.0
-0.2
-0.2
+0.4
stress
Chlorite 0.08 mM
+0.5
-0.1
-0.2
+0.1
+0.3
stress
Rifampicin 0.0024 mg/ml
+0.6
-0.1
-0.0
-0.2
+0.3
stress
KB-half, Pseudo5 supernatant
-0.6
-0.3
+0.4
+0.4
+0.8
stress
Vanillin 1.25 mM
+0.5
+0.1
+0.3
+0.1
+0.3
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