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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_24990 and AO356_24995 are separated by 128 nucleotides
AO356_24995 and AO356_25000 are separated by 51 nucleotides
AO356_25000 and AO356_25005 are separated by 320 nucleotides
AO356_25005 and AO356_25010 are separated by 90 nucleotides
AO356_24990: AO356_24990 - 4-oxalocrotonate tautomerase, at 5,662,614 to 5,662,820
_24990
AO356_24995: AO356_24995 - LysR family transcriptional regulator, at 5,662,949 to 5,663,872
_24995
AO356_25000: AO356_25000 - hypothetical protein, at 5,663,924 to 5,664,358
_25000
AO356_25005: AO356_25005 - LysR family transcriptional regulator, at 5,664,679 to 5,665,575
_25005
AO356_25010: AO356_25010 - MFS transporter, at 5,665,666 to 5,667,081
_25010
Group
Condition
AO356
_24990
AO356
_24995
AO356
_25000
AO356
_25005
AO356
_25010
stress
Chlorite 0.08 mM
-2.2
-0.0
-0.6
+0.7
-0.3
survival
4C survival; 1152 hrs
-1.5
-0.1
-0.4
-0.2
+0.0
survival
4C survival; 672 hrs
-1.1
-0.5
+0.2
-0.1
-0.2
stress
Sisomicin 0.001 mg/ml
-0.7
-0.6
+0.3
-0.2
-0.4
pH
Minimal media pH6
-0.5
-0.3
-0.4
-0.3
-0.2
resistance
10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-1.1
-0.2
-0.4
+0.1
+0.2
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-1.1
+0.0
-0.2
-0.1
+0.2
stress
Benzalkonium Chloride 0.008 mg/ml
-0.7
-0.2
-0.5
+0.3
+0.0
motility
outer cut, LB soft agar motility assay
+0.1
-0.4
-0.6
-0.5
+0.3
anaerobic
Nitrate reduction with yeast extract
+0.0
-0.9
-0.4
+0.1
+0.2
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-1.0
+0.1
-0.2
+0.1
+0.2
stress
nitrate 200 mM
-0.4
-0.3
-0.6
+0.2
+0.3
carbon source
Carbon source Sucrose 2.5 mM
-0.7
-0.3
+0.2
+0.4
-0.4
stress
Lomefloxacin 0.0005 mM
+0.2
+0.0
-1.3
+0.6
-0.1
carbon source
Carbon source D-Glucose 10 mM
-0.8
-0.1
+0.4
+0.1
-0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.8
-0.2
-0.1
+0.6
+0.1
carbon source
Carbon source D-Mannitol 5 mM
-0.9
-0.2
+0.2
+0.3
+0.1
carbon source
Carbon source Sucrose 10 mM
-0.8
-0.4
+0.4
+0.4
+0.0
carbon source
Carbon source Sucrose 2.5 mM
-0.8
-0.3
+0.4
+0.4
-0.0
carbon source
Carbon source D-Mannitol 2.5 mM
-0.9
+0.1
+0.1
+0.6
-0.1
carbon source
Carbon source D-Mannitol 5 mM
-0.2
-0.6
+0.3
+0.5
+0.0
stress
methylglyoxal 0.016 vol%
-0.1
+0.6
-0.2
-0.5
+0.4
carbon source
Carbon source Sucrose 5 mM
-0.3
-0.3
+0.6
+0.4
-0.2
carbon source
Carbon source Sucrose 5 mM
+0.0
-0.4
+0.7
+0.2
-0.3
motility
outer cut, LB soft agar motility assay
-0.2
+0.2
+0.3
-0.6
+0.6
stress
copper (II) chloride 2.5 mM
+0.5
-0.8
+0.5
-0.2
+0.3
carbon source
D-Mannose (C)
+0.7
+0.1
+0.1
+0.2
-0.5
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.1
+0.1
+0.5
+0.8
-0.4
stress
KB-half, Pseudo5 supernatant
+1.2
-0.2
+0.5
-0.1
-0.2
nitrogen source
D-Serine (N)
+1.1
+0.4
-0.3
+0.2
+1.3
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