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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_24800 and AO356_24805 are separated by 509 nucleotides
AO356_24805 and AO356_24810 are separated by 214 nucleotides
AO356_24810 and AO356_24815 are separated by 196 nucleotides
AO356_24815 and AO356_24820 are separated by 340 nucleotides
AO356_24800: AO356_24800 - type II secretion system protein GspF, at 5,607,933 to 5,609,144
_24800
AO356_24805: AO356_24805 - alkaline phosphatase, at 5,609,654 to 5,610,823
_24805
AO356_24810: AO356_24810 - hemagglutinin, at 5,611,038 to 5,623,655
_24810
AO356_24815: AO356_24815 - diguanylate cyclase, at 5,623,852 to 5,626,425
_24815
AO356_24820: AO356_24820 - calcium-binding protein, at 5,626,766 to 5,627,152
_24820
Group
Condition
AO356
_24800
AO356
_24805
AO356
_24810
AO356
_24815
AO356
_24820
anaerobic
Nitrate reduction with yeast extract
-1.0
+0.1
+0.1
+0.3
-1.0
carbon source
succinate (C)
-0.2
-0.0
-0.0
+0.1
-1.2
nitrogen source
L-Phenylalanine (N)
-0.2
-0.1
+0.1
-0.2
-0.9
stress
syringaldehyde 5 mM
+0.0
-0.4
+0.0
-0.2
-0.6
motility
outer cut, LB soft agar motility assay
-0.3
-0.3
-0.0
+0.2
-0.7
carbon source
D-Fructose (C)
-0.0
-0.3
+0.0
+0.1
-0.8
stress
Cobalt chloride 0.08 mM
-0.2
+0.2
+0.0
-0.0
-0.8
stress
copper (II) chloride 2.5 mM
+0.2
-0.0
+0.1
+0.4
-1.6
carbon source
D-Glucose (C)
-0.3
+0.1
-0.0
+0.2
-0.8
stress
Benzalkonium Chloride 0.016 mg/ml
+0.3
+0.1
+0.1
+0.5
-1.7
stress
Chlorite 0.08 mM
+0.1
-0.0
+0.0
+0.2
-1.0
carbon source
L-Leucine (C)
+0.0
+0.3
+0.0
-0.1
-0.8
stress
Lomefloxacin 0.0005 mM
-0.6
-0.3
+0.2
-0.5
+0.7
stress
Cobalt chloride 0.08 mM
-0.3
+0.2
+0.0
+0.2
-0.4
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.2
-0.5
+0.0
+0.1
+0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.2
+0.0
-0.5
-0.4
+0.4
stress
Nickel (II) chloride 0.625 mM
-0.0
+0.2
-0.1
+0.2
-0.6
motility
outer cut, LB soft agar motility assay
-0.2
-0.7
+0.2
+0.3
+0.2
carbon source
a-Ketoglutaric (C)
-0.1
+0.3
-0.0
+0.2
-0.5
stress
Doxycycline hyclate 0.0001 mg/ml
+0.1
+0.4
-0.0
+0.1
-0.7
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.4
-0.1
-0.1
-0.1
+0.5
carbon source
Cytidine (C)
-0.3
-0.3
+0.1
+0.4
+0.2
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.1
+0.2
-0.0
+0.4
-0.4
stress
KB-half, Pseudo5 supernatant
+0.4
+0.4
-0.4
-0.1
+0.0
nitrogen source
Uridine (N)
-0.4
+0.1
+0.0
+0.2
+0.5
lb
LB
+0.3
+0.7
+0.0
-0.2
-0.1
stress
Cephalothin 2 mg/ml
-0.1
+0.2
-0.1
+0.2
+0.6
motility
outer cut, LB soft agar motility assay
-0.3
+0.3
+0.1
+0.1
+0.6
pH
Minimal media pH7
+0.4
+0.0
-0.1
+0.1
+0.6
carbon source
Carbon source Sucrose 5 mM
+0.3
+0.2
+0.1
+0.3
+0.5
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