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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_24405 and AO356_24410 are separated by 123 nucleotides
AO356_24410 and AO356_24415 are separated by 182 nucleotides
AO356_24415 and AO356_24420 are separated by 23 nucleotides
AO356_24420 and AO356_24425 are separated by 146 nucleotides
AO356_24405: AO356_24405 - tRNA hydroxylase, at 5,512,165 to 5,512,770
_24405
AO356_24410: AO356_24410 - alanine acetyltransferase, at 5,512,894 to 5,513,382
_24410
AO356_24415: AO356_24415 - DNA-3-methyladenine glycosylase, at 5,513,565 to 5,514,182
_24415
AO356_24420: AO356_24420 - XRE family transcriptional regulator, at 5,514,206 to 5,515,288
_24420
AO356_24425: AO356_24425 - hypothetical protein, at 5,515,435 to 5,515,923
_24425
Group
Condition
AO356
_24405
AO356
_24410
AO356
_24415
AO356
_24420
AO356
_24425
anaerobic
Nitrate reduction with yeast extract
-1.7
-0.8
+0.5
-0.5
N.D.
motility
outer cut, LB soft agar motility assay
-1.4
+0.0
-0.8
-0.2
N.D.
temperature
LB 30C
-0.3
-0.5
-0.4
-0.3
N.D.
temperature
LB 10C
-0.3
-0.6
+0.0
-0.4
N.D.
stress
Chlorite 0.08 mM
-0.9
+0.0
-0.1
-0.3
N.D.
stress
Chlorite 0.04 mM
-0.0
-0.3
+0.2
-1.1
N.D.
carbon source
D-Trehalose (C)
-0.6
-0.3
-0.4
+0.2
N.D.
carbon source
L-Lysine (C)
-0.2
-0.9
-0.2
+0.1
N.D.
stress
Sisomicin 0.001 mg/ml
+0.4
-1.1
-0.1
+0.1
N.D.
stress
methylglyoxal 0.016 vol%
+0.4
-0.5
+0.1
-0.6
N.D.
stress
copper (II) chloride 2.5 mM
-0.2
+1.2
-0.7
-0.8
N.D.
stress
Cholin acetate 40 mM
+0.7
-0.3
-0.5
-0.2
N.D.
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.0
+0.6
-0.9
-0.0
N.D.
stress
Lomefloxacin 0.0005 mM
+0.5
-1.2
+0.2
+0.6
N.D.
stress
1-ethyl-3-methylimidazolium acetate 10 mM
+0.6
-1.0
+0.2
+0.3
N.D.
stress
Ceftazidime 0.2 mg/ml
+0.4
-0.4
-0.1
+0.4
N.D.
stress
Benzalkonium Chloride 0.016 mg/ml
+0.7
-0.6
-0.1
+0.3
N.D.
survival
4C survival; 360 hrs
+0.5
-0.5
+0.3
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.4
+0.7
-1.1
+0.4
N.D.
carbon source
Carbon source D,L-Lactate 10 mM
+0.5
+0.3
+0.2
-0.4
N.D.
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.6
+0.4
-0.2
-0.2
N.D.
carbon source
D-Mannose (C)
-0.1
+0.8
+0.3
-0.4
N.D.
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.5
+0.5
-0.2
-0.1
N.D.
nitrogen source
D-Alanine (N)
+0.1
+0.6
+0.4
-0.3
N.D.
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.6
-0.2
+0.2
+0.4
N.D.
stress
nitrite 10 mM
-0.4
+1.0
+0.6
-0.2
N.D.
stress
nitrate 200 mM
+0.2
-0.2
+0.7
+0.4
N.D.
stress
Cholin acetate 20 mM
+0.5
+0.3
+0.7
-0.3
N.D.
carbon source
Cytidine (C)
+0.3
+1.2
-0.1
-0.0
N.D.
carbon source
Inosine (C)
+0.2
+0.6
+0.4
+0.1
N.D.
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