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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_24205 and AO356_24210 are separated by 158 nucleotides
AO356_24210 and AO356_24215 are separated by 3 nucleotides
AO356_24215 and AO356_24220 are separated by 355 nucleotides
AO356_24220 and AO356_24225 are separated by 8 nucleotides
AO356_24205: AO356_24205 - Alpha,alpha-trehalase (EC 3.2.1.28)
(from data)
, at 5,428,153 to 5,429,778
_24205
AO356_24210: AO356_24210 - diguanylate cyclase, at 5,429,937 to 5,430,890
_24210
AO356_24215: AO356_24215 - chemotaxis protein CheC, at 5,430,894 to 5,431,904
_24215
AO356_24220: AO356_24220 - diguanylate cyclase, at 5,432,260 to 5,434,632
_24220
AO356_24225: AO356_24225 - phosphate ABC transporter substrate-binding protein, at 5,434,641 to 5,435,078
_24225
Group
Condition
AO356
_24205
AO356
_24210
AO356
_24215
AO356
_24220
AO356
_24225
carbon source
D-Trehalose (C)
-4.9
+1.0
+0.4
+0.4
+0.3
carbon source
D-Trehalose (C)
-4.7
+1.0
+0.4
+0.4
+0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
-0.0
-1.4
-0.3
+0.3
anaerobic
Nitrate reduction with yeast extract
+0.2
-1.0
-0.1
-0.1
-0.0
stress
copper (II) chloride 2.5 mM
+0.1
-0.1
-0.9
-0.0
-0.2
stress
Chlorite 0.08 mM
-0.2
-0.0
-0.1
-0.3
-0.5
stress
KB-half, Pseudo5 supernatant
+0.0
-0.1
-1.0
-0.3
+0.3
stress
Choline chloride 300 mM
-0.1
-0.1
-0.5
+0.0
-0.2
stress
Lomefloxacin 0.0005 mM
-0.1
+0.0
-0.9
+0.1
+0.1
nitrogen source
Gly-Glu (N)
+0.1
+0.0
-0.4
-0.2
-0.2
stress
syringaldehyde 5 mM
+0.0
+0.3
-0.8
+0.1
-0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.2
-0.2
-0.1
-0.5
+0.4
motility
outer cut, LB soft agar motility assay
-0.0
-0.1
+0.2
+0.1
-0.6
carbon source
a-Ketoglutaric (C)
+0.1
-0.0
-0.5
-0.2
+0.2
carbon source
acetate (C)
-0.1
+0.2
+0.1
-0.1
-0.4
carbon source
L-Lysine (C)
+0.1
+0.3
-0.0
+0.2
-0.6
carbon source
L-Lactate (C)
-0.1
-0.1
+0.5
+0.0
-0.3
carbon source
Cytidine (C)
+0.0
-0.2
+0.4
-0.2
+0.2
stress
Cholin acetate 20 mM
-0.0
+0.2
-0.6
+0.1
+0.5
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.1
+0.1
-0.4
+0.2
+0.4
carbon source
L-Arginine (C)
+0.1
-0.0
+0.2
+0.3
-0.4
nitrogen source
L-Leucine (N)
+0.0
+0.2
+0.4
-0.2
-0.2
carbon source
Carbon source D-Mannitol 2.5 mM
-0.1
-0.2
+0.3
-0.1
+0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.1
+0.2
-0.5
+0.2
+0.4
stress
Thallium(I) acetate 0.008 mg/ml
-0.0
+0.3
+0.4
-0.0
-0.2
survival
4C survival; 672 hrs
-0.0
+0.1
+0.5
-0.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.0
+0.5
+0.2
-0.1
+0.2
carbon source
Carbon source D-Glucose 10 mM
-0.1
-0.0
+0.5
+0.2
+0.4
carbon source
Carbon source Sucrose 10 mM
+0.0
-0.0
+0.6
+0.2
+0.1
motility
outer cut, LB soft agar motility assay
-0.0
+0.6
+0.5
+0.3
+0.2
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