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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_23885 and AO356_23890 are separated by 59 nucleotides
AO356_23890 and AO356_23895 are separated by 257 nucleotides
AO356_23895 and AO356_23900 are separated by 100 nucleotides
AO356_23900 and AO356_23905 are separated by 51 nucleotides
AO356_23885: AO356_23885 - carbonate dehydratase, at 5,352,877 to 5,355,624
_23885
AO356_23890: AO356_23890 - universal stress protein, at 5,355,684 to 5,356,607
_23890
AO356_23895: AO356_23895 - hypothetical protein, at 5,356,865 to 5,358,115
_23895
AO356_23900: AO356_23900 - universal stress protein, at 5,358,216 to 5,359,127
_23900
AO356_23905: AO356_23905 - GNAT family acetyltransferase, at 5,359,179 to 5,359,742
_23905
Group
Condition
AO356
_23885
AO356
_23890
AO356
_23895
AO356
_23900
AO356
_23905
stress
Sisomicin 0.001 mg/ml
+0.0
-0.0
-0.5
-0.6
-0.1
pH
Minimal media pH7
+0.0
-0.2
-0.2
-0.3
-0.2
temperature
LB 30C
+0.1
-0.1
-0.4
-0.1
-0.3
carbon source
L-Arabinose (C)
-0.1
-0.1
-0.3
-0.4
+0.1
stress
Nickel (II) chloride 0.625 mM
-0.1
-0.1
-0.3
-0.3
+0.1
stress
Cholin acetate 20 mM
+0.1
+0.1
-0.3
-0.3
-0.3
stress
nitrite 10 mM
-0.0
+0.3
-0.4
-0.2
-0.4
motility
outer cut, LB soft agar motility assay
+0.2
-0.3
-0.1
+0.0
-0.5
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.2
+0.1
-0.5
-0.2
-0.3
pH
Minimal media pH8
-0.2
-0.0
-0.1
+0.2
-0.3
stress
Chlorite 0.08 mM
-0.1
-0.2
-0.0
-0.5
+0.3
stress
Benzalkonium Chloride 0.008 mg/ml
-0.0
-0.5
+0.1
-0.3
+0.3
nitrogen source
L-Leucine (N)
-0.1
-0.1
-0.2
+0.3
-0.4
carbon source
L-Arabinose (C)
-0.1
-0.0
-0.3
-0.2
+0.4
motility
outer cut, LB soft agar motility assay
+0.2
+0.1
-0.7
+0.2
-0.1
carbon source
Carbon source Sucrose 10 mM
+0.0
+0.0
+0.0
+0.3
-0.6
stress
Lomefloxacin 0.0005 mM
-0.0
+0.5
-0.1
-0.5
+0.1
carbon source
acetate (C)
+0.0
-0.2
-0.3
+0.0
+0.4
stress
sodium fluoride 6.25 mM
-0.0
-0.2
+0.3
-0.2
+0.2
stress
Chloramphenicol 0.005 mg/ml
-0.0
-0.2
+0.2
-0.1
+0.3
motility
outer cut, LB soft agar motility assay
+0.1
+0.2
+0.2
+0.2
-0.3
carbon source
D-Galactose (C)
+0.0
-0.2
-0.1
+0.2
+0.4
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.0
-0.3
+0.1
-0.2
+0.7
stress
Cobalt chloride 0.08 mM
+0.1
-0.3
+0.1
+0.2
+0.3
nitrogen source
Carnitine Hydrochloride (N)
+0.0
+0.2
-0.2
+0.3
+0.3
carbon source
L-Glutamine (C)
-0.1
+0.2
-0.1
+0.2
+0.4
stress
copper (II) chloride 2.5 mM
-0.3
+0.3
+0.4
+0.1
+0.4
anaerobic
Nitrate reduction with yeast extract
+0.3
+0.4
-0.1
+0.1
+1.0
stress
KB-half, Pseudo5 supernatant
+0.1
+0.4
+1.3
+0.1
-0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.3
+0.4
+0.8
+0.3
+0.4
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