Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_23465 and AO356_23470 are separated by 58 nucleotidesAO356_23470 and AO356_23475 are separated by 74 nucleotidesAO356_23475 and AO356_23480 are separated by 119 nucleotidesAO356_23480 and AO356_23485 are separated by 3 nucleotides AO356_23465: AO356_23465 - polyketide cyclase, at 5,260,042 to 5,260,512 _23465 AO356_23470: AO356_23470 - polyketide synthase, at 5,260,571 to 5,263,981 _23470 AO356_23475: AO356_23475 - polyketide synthase, at 5,264,056 to 5,265,147 _23475 AO356_23480: AO356_23480 - FAD-binding monooxygenase, at 5,265,267 to 5,267,030 _23480 AO356_23485: AO356_23485 - haloacid dehalogenase, at 5,267,034 to 5,267,645 _23485
Group Condition AO356_23465 AO356_23470 AO356_23475 AO356_23480 AO356_23485
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 N.D. -0.1 -0.8 -0.2 -0.5
motility outer cut, LB soft agar motility assay N.D. -0.2 -0.7 +0.1 -0.4
nitrogen source Thymine (N) N.D. -0.2 -0.6 -0.1 -0.1
stress copper (II) chloride 2.5 mM N.D. -0.4 -0.6 -0.0 +0.0
stress Chloramphenicol 0.005 mg/ml N.D. -0.2 -0.5 +0.0 -0.2
survival 4C survival; 1152 hrs N.D. -0.3 -0.2 -0.1 -0.3
stress KB-half, Pseudo5 supernatant N.D. -0.1 -0.3 -0.1 -0.4
survival 4C survival; 672 hrs N.D. -0.3 -0.1 -0.2 -0.3
anaerobic Nitrate reduction with yeast extract N.D. -0.0 -0.4 -0.5 +0.2
motility outer cut, LB soft agar motility assay N.D. -0.1 -0.3 +0.2 -0.5
carbon source L-Asparagine (C) N.D. -0.1 -0.5 +0.1 -0.2
carbon source D-Glucosamine Hydrochloride (C) N.D. -0.1 -0.7 +0.1 +0.0
carbon source L-Arginine (C) N.D. -0.1 -0.7 +0.1 +0.1
stress Cisplatin 0.0032 mg/ml N.D. +0.0 -0.8 +0.1 +0.0
carbon source Fumarate (C) N.D. +0.1 -0.6 -0.1 +0.1
stress Cobalt chloride 0.08 mM N.D. -0.1 -0.5 -0.1 +0.3
motility outer cut, LB soft agar motility assay N.D. -0.1 +0.4 +0.0 -0.8
carbon source L-Arabinose (C) N.D. +0.0 -0.4 -0.3 +0.2
stress acetate 40 mM N.D. -0.3 +0.3 -0.1 -0.2
nitrogen source D-Serine (N) N.D. -0.1 -0.7 +0.1 +0.4
pH Minimal media pH8 N.D. -0.1 -0.4 +0.2 +0.1
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine N.D. +0.2 -0.5 +0.1 +0.2
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. +0.0 -0.3 -0.2 +0.4
stress methylglyoxal 0.016 vol% N.D. -0.1 +0.5 -0.3 -0.1
stress 1-ethyl-3-methylimidazolium acetate 10 mM N.D. -0.1 +0.5 +0.3 -0.4
carbon source L-Histidine (C) N.D. -0.0 -0.3 +0.0 +0.5
carbon source L-Histidine (C) N.D. -0.1 -0.3 +0.0 +0.6
stress Aluminum chloride 5 mM N.D. +0.2 -0.3 +0.0 +0.4
carbon source acetate (C) N.D. +0.2 -0.2 +0.2 +0.3
carbon source D-Fructose (C) N.D. +0.2 +0.1 +0.2 +0.4
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