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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_23430 and AO356_23435 are separated by 14 nucleotides
AO356_23435 and AO356_23440 overlap by 8 nucleotides
AO356_23440 and AO356_23445 are separated by 245 nucleotides
AO356_23445 and AO356_23450 are separated by 70 nucleotides
AO356_23430: AO356_23430 - NAD-dependent deacetylase, at 5,254,131 to 5,254,886
_23430
AO356_23435: AO356_23435 - hypothetical protein, at 5,254,901 to 5,255,380
_23435
AO356_23440: AO356_23440 - calpastatin, at 5,255,373 to 5,255,801
_23440
AO356_23445: AO356_23445 - hypothetical protein, at 5,256,047 to 5,256,829
_23445
AO356_23450: AO356_23450 - LuxR family transcriptional regulator, at 5,256,900 to 5,257,481
_23450
Group
Condition
AO356
_23430
AO356
_23435
AO356
_23440
AO356
_23445
AO356
_23450
stress
KB-half, Pseudo5 supernatant
-0.3
-0.6
-0.2
-0.9
+0.2
survival
4C survival; 1152 hrs
-0.1
+0.0
-1.0
-0.3
-0.1
motility
outer cut, LB soft agar motility assay
-1.1
-0.9
-0.6
+0.6
+0.5
stress
Chlorite 0.08 mM
-0.0
-0.4
-0.2
-0.9
-0.0
pH
Minimal media pH8
-0.3
-0.2
-0.4
-0.5
-0.0
pH
Minimal media pH9
+0.2
-0.3
-1.1
-0.2
+0.1
stress
Sisomicin 0.001 mg/ml
+0.2
-0.5
-0.6
-0.5
+0.2
motility
outer cut, LB soft agar motility assay
-0.4
-0.5
+0.3
+0.0
-0.5
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.6
+0.5
-0.1
-0.5
-0.2
anaerobic
Nitrate reduction with yeast extract
+0.8
-0.7
-0.7
+0.2
-0.3
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.7
+0.1
-0.2
-0.4
+0.4
survival
4C survival; 192 hrs
+0.1
+0.1
-0.8
-0.2
+0.2
stress
Rifampicin 0.0016 mg/ml
-0.3
+0.3
-0.1
-0.3
-0.3
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.2
+0.5
+0.1
-0.7
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.6
-0.6
-0.2
-0.2
carbon source
Carbon source Sucrose 5 mM
+0.1
-0.3
+0.3
-0.6
-0.0
nitrogen source
Gly-Glu (N)
-0.2
+0.1
-0.2
-0.4
+0.4
carbon source
D-Galactose (C)
+0.2
-0.2
-0.3
-0.4
+0.3
stress
Nickel (II) chloride 0.625 mM
-0.1
+0.4
-0.4
-0.4
+0.2
motility
outer cut, LB soft agar motility assay
+0.8
-0.3
-0.7
+0.1
-0.2
carbon source
Carnitine Hydrochloride (C)
+0.3
-0.6
+0.2
-0.1
+0.1
nitrogen source
D-Serine (N)
+0.0
-0.1
+0.6
-0.1
-0.6
stress
Paraquat dichloride 0.0625 mg/ml
+0.3
+0.6
-0.3
-0.5
-0.2
nitrogen source
L-Phenylalanine (N)
+0.1
+0.4
-0.1
-0.5
+0.3
stress
Benzalkonium Chloride 0.016 mg/ml
+0.3
+0.4
+0.2
-0.6
+0.0
carbon source
L-Lactate (C)
+0.4
+0.5
-0.1
-0.3
-0.0
carbon source
L-Arabinose (C)
+0.2
+0.2
+0.3
-0.5
+0.3
nitrogen source
L-tyrosine (N)
+0.2
+0.6
+0.2
-0.5
+0.2
stress
Lomefloxacin 0.0005 mM
+0.7
-0.0
+0.3
+0.5
+0.1
stress
copper (II) chloride 2.5 mM
+0.6
+1.0
-0.0
-0.2
+0.6
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