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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22970 and AO356_22975 are separated by 257 nucleotides
AO356_22975 and AO356_22980 are separated by 3 nucleotides
AO356_22980 and AO356_22985 are separated by 0 nucleotides
AO356_22985 and AO356_22990 are separated by 1 nucleotides
AO356_22970: AO356_22970 - Branched-chain amino acid aminotransferase (EC 2.6.1.42)
(from data)
, at 5,127,612 to 5,128,631
_22970
AO356_22975: AO356_22975 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4)
(from data)
, at 5,128,889 to 5,130,274
_22975
AO356_22980: AO356_22980 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 5,130,278 to 5,131,546
_22980
AO356_22985: AO356_22985 - branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit (EC 1.2.4.4)
(from data)
, at 5,131,547 to 5,132,605
_22985
AO356_22990: AO356_22990 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4)
(from data)
, at 5,132,607 to 5,133,842
_22990
Group
Condition
AO356
_22970
AO356
_22975
AO356
_22980
AO356
_22985
AO356
_22990
carbon source
L-Isoleucine (C)
-5.7
-6.2
-5.3
-5.0
-5.9
carbon source
L-Leucine (C)
-4.2
-2.5
-6.1
-5.8
-5.4
nitrogen source
L-Leucine (N)
-4.2
-1.0
-1.6
-1.5
-1.4
nitrogen source
L-Isoleucine (N)
-4.1
-1.2
-1.9
-1.3
-1.1
carbon source
acetate (C)
-2.0
-0.1
-1.7
-0.8
-0.9
carbon source
acetate (C)
-1.0
+0.1
-1.1
-0.6
-0.9
temperature
LB 30C
-0.8
-0.2
-1.0
-0.6
-0.6
carbon source
L-Lysine (C)
-2.5
-0.2
-0.1
+0.1
-0.3
carbon source
L-Lysine (C)
-2.3
-0.6
+0.2
+0.0
-0.3
carbon source
casaminos (C)
-0.8
-0.4
-0.6
-0.4
-0.7
stress
nitrate 200 mM
-0.3
-0.3
-1.2
+0.0
-0.4
temperature
LB 30C
-0.4
-0.1
-0.2
-0.4
-0.8
carbon source
D-Alanine (C)
-0.6
+0.1
+0.4
-0.3
-1.5
anaerobic
Nitrate reduction with yeast extract
+0.0
+0.3
-2.4
+0.3
-0.2
motility
outer cut, LB soft agar motility assay
-1.0
-0.6
+0.1
-0.3
-0.0
stress
Cholin acetate 40 mM
+0.1
+0.1
-2.0
-0.0
+0.1
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.1
-0.0
-0.6
-0.3
-0.6
stress
Choline chloride 300 mM
-0.3
-0.2
-1.0
-0.2
-0.0
carbon source
L-Alanine (C)
-0.2
-0.2
-0.5
+0.0
-0.8
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.2
+0.1
-0.7
-0.4
-0.3
carbon source
D-Xylose (C)
-0.4
-0.1
-0.6
-0.1
-0.2
carbon source
m-Inositol (C)
-0.7
-0.3
-0.5
+0.2
-0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.8
+0.2
-0.2
-0.2
-0.4
carbon source
L-Histidine (C)
-0.5
-0.2
-0.9
+0.2
+0.1
carbon source
L-Histidine (C)
-0.5
-0.2
-0.9
+0.2
+0.1
stress
Sisomicin 0.001 mg/ml
-1.2
+0.2
+0.1
+0.3
-0.1
motility
outer cut, LB soft agar motility assay
-0.7
+0.3
+0.1
+0.2
-0.5
stress
KB-half, Pseudo5 supernatant
-0.0
+0.5
-0.9
+0.4
+0.1
survival
4C survival; 1152 hrs
+0.1
-0.5
+0.3
+0.4
+0.3
carbon source
Carbon source D-Mannitol 10 mM
+0.3
+0.3
+0.7
-0.1
+0.2
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