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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22875 and AO356_22880 are separated by 41 nucleotides
AO356_22880 and AO356_22885 are separated by 371 nucleotides
AO356_22885 and AO356_22890 are separated by 95 nucleotides
AO356_22890 and AO356_22895 are separated by 222 nucleotides
AO356_22875: AO356_22875 - chemotaxis protein, at 5,105,575 to 5,107,200
_22875
AO356_22880: AO356_22880 - aspartate aminotransferase, at 5,107,242 to 5,108,429
_22880
AO356_22885: AO356_22885 - hypothetical protein, at 5,108,801 to 5,109,604
_22885
AO356_22890: AO356_22890 - hypothetical protein, at 5,109,700 to 5,111,709
_22890
AO356_22895: AO356_22895 - hypothetical protein, at 5,111,932 to 5,114,172
_22895
Group
Condition
AO356
_22875
AO356
_22880
AO356
_22885
AO356
_22890
AO356
_22895
anaerobic
Nitrate reduction with yeast extract
+0.2
-0.5
-1.8
+0.1
-0.3
stress
Chlorite 0.04 mM
-1.0
-0.2
-0.5
+0.0
+0.0
survival
4C survival; 1152 hrs
-0.6
+0.1
-0.4
-0.3
-0.1
stress
Benzalkonium Chloride 0.016 mg/ml
-0.2
-0.2
-0.9
-0.2
+0.1
survival
4C survival; 360 hrs
-0.5
-0.2
-0.2
-0.4
-0.0
phage
P5ss-5 phage 10-1 dilution SM calcl2
+0.1
-0.0
-1.0
-0.2
-0.1
nitrogen source
Uridine (N)
-0.2
-0.2
-0.4
-0.3
-0.0
motility
outer cut, LB soft agar motility assay
-0.6
-0.0
-0.2
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
-0.5
-0.2
-0.5
-0.0
+0.2
pH
Minimal media pH6
-0.6
-0.2
-0.0
-0.0
+0.0
temperature
LB 10C
+0.0
-0.2
-0.5
-0.1
-0.0
carbon source
Carbon source D-Mannitol 10 mM
-0.1
+0.1
-0.6
-0.1
-0.0
carbon source
Carbon source Sucrose 10 mM
+0.1
+0.1
-0.6
-0.2
-0.1
pH
Minimal media pH8
-0.4
+0.1
-0.6
+0.2
+0.1
stress
copper (II) chloride 2.5 mM
-0.4
-0.1
-0.3
+0.3
+0.1
motility
outer cut, LB soft agar motility assay
+0.3
-0.4
-0.2
-0.2
+0.2
carbon source
L-Asparagine (C)
-0.4
-0.2
+0.3
-0.1
+0.1
carbon source
m-Inositol (C)
-0.2
-0.3
+0.3
-0.1
+0.0
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
-0.2
+0.6
-0.3
-0.2
-0.1
stress
Sisomicin 0.001 mg/ml
-0.2
-0.2
+0.3
+0.2
-0.0
stress
Cobalt chloride 0.08 mM
-0.2
-0.2
+0.3
+0.1
+0.1
stress
Lomefloxacin 0.0005 mM
+0.0
+0.1
+0.6
-0.4
-0.1
stress
KB-half, Pseudo5 supernatant
-0.3
+0.6
+0.3
-0.2
-0.2
carbon source
L-Arginine (C)
-0.2
-0.1
+0.3
+0.2
+0.1
stress
Nickel (II) chloride 0.625 mM
+0.4
-0.2
+0.0
+0.2
+0.0
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
-0.1
+0.1
+0.6
-0.1
-0.0
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.2
+0.1
+0.8
-0.2
+0.0
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.0
+0.1
+0.6
-0.2
+0.0
nitrogen source
L-Isoleucine (N)
+0.1
-0.0
+0.5
+0.2
+0.0
stress
methylglyoxal 0.016 vol%
+0.1
+0.1
+0.4
+0.4
+0.0
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