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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22765 and AO356_22770 are separated by 388 nucleotides
AO356_22770 and AO356_22775 are separated by 202 nucleotides
AO356_22775 and AO356_22780 are separated by 109 nucleotides
AO356_22780 and AO356_22785 are separated by 30 nucleotides
AO356_22765: AO356_22765 - proline hydroxylase, at 5,083,473 to 5,084,201
_22765
AO356_22770: AO356_22770 - hypothetical protein, at 5,084,590 to 5,084,892
_22770
AO356_22775: AO356_22775 - hypothetical protein, at 5,085,095 to 5,085,445
_22775
AO356_22780: AO356_22780 - hypothetical protein, at 5,085,555 to 5,085,785
_22780
AO356_22785: AO356_22785 - hypothetical protein, at 5,085,816 to 5,086,097
_22785
Group
Condition
AO356
_22765
AO356
_22770
AO356
_22775
AO356
_22780
AO356
_22785
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.0
-1.3
-1.0
+0.4
-0.4
carbon source
L-Lysine (C)
-0.3
-0.8
-0.3
-0.3
-0.1
carbon source
D-Xylose (C)
-0.2
-0.4
-0.2
+0.1
-1.1
stress
perchlorate 75 mM
-0.3
-0.2
-0.9
-0.2
-0.2
stress
KB-half, Pseudo5 supernatant
-0.1
-1.4
-0.6
+0.3
+0.2
carbon source
L-Lysine (C)
+0.1
-0.6
-1.4
-0.1
+0.3
carbon source
L-Arabinose (C)
-0.1
-0.2
-0.5
+0.1
-0.7
motility
outer cut, LB soft agar motility assay
-0.5
-0.4
+0.3
-0.4
-0.4
carbon source
D,L-Lactate (C)
-0.0
-0.3
-0.5
+0.0
-0.5
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.1
-0.4
-0.4
-0.1
-0.3
stress
methylglyoxal 0.016 vol%
-0.2
-0.1
+0.2
+0.0
-0.8
motility
outer cut, LB soft agar motility assay
-0.3
-0.4
+0.3
+0.1
-0.6
stress
Chlorite 0.04 mM
-0.1
+0.2
-0.6
+0.0
-0.4
nitrogen source
L-tyrosine (N)
+0.1
+0.2
-1.0
-0.1
+0.0
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.2
+0.2
-0.9
-0.0
+0.2
nitrogen source
D-Serine (N)
-0.0
+0.2
-0.6
-0.1
-0.3
carbon source
L-Isoleucine (C)
+0.1
+0.2
-0.9
-0.1
+0.2
stress
copper (II) chloride 2.5 mM
-0.2
+1.3
-1.2
-0.5
+0.0
stress
Chlorite 0.08 mM
-0.2
+0.2
-0.5
-0.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.4
-0.3
+1.2
-0.3
-0.7
carbon source
L-Asparagine (C)
+0.1
-0.5
+0.3
+0.1
-0.3
pH
Minimal media pH8
-0.1
-0.1
-0.5
-0.1
+0.5
stress
sodium fluoride 6.25 mM
-0.1
+0.3
+0.3
-0.2
-0.5
stress
Cholin acetate 40 mM
+0.0
+0.3
+0.2
+0.1
-0.8
carbon source
Carbon source D-Mannitol 2.5 mM
-0.2
-0.5
+0.1
+0.3
+0.3
stress
Bacitracin 2 mg/ml
-0.1
+0.2
+0.6
+0.1
-0.6
survival
4C survival; 1152 hrs
+0.2
-0.1
+0.8
-0.1
-0.5
anaerobic
Nitrate reduction with yeast extract
-0.3
+0.5
-0.3
+0.2
+0.4
stress
Lomefloxacin 0.0005 mM
+0.1
+0.6
+0.8
+0.4
+0.5
stress
Benzalkonium Chloride 0.016 mg/ml
+0.2
+0.3
+1.7
+0.6
-0.1
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