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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22650 and AO356_22655 overlap by 4 nucleotides
AO356_22655 and AO356_22660 overlap by 4 nucleotides
AO356_22660 and AO356_22665 are separated by 39 nucleotides
AO356_22665 and AO356_22670 are separated by 33 nucleotides
AO356_22650: AO356_22650 - chemotaxis protein CheD, at 5,061,794 to 5,062,291
_22650
AO356_22655: AO356_22655 - SAM-dependent methyltransferase, at 5,062,288 to 5,063,124
_22655
AO356_22660: AO356_22660 - chemotaxis protein CheW, at 5,063,121 to 5,063,657
_22660
AO356_22665: AO356_22665 - chemotaxis protein, at 5,063,697 to 5,065,355
_22665
AO356_22670: AO356_22670 - chemotaxis protein CheA, at 5,065,389 to 5,067,491
_22670
Group
Condition
AO356
_22650
AO356
_22655
AO356
_22660
AO356
_22665
AO356
_22670
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-1.8
+0.0
+0.1
-0.6
+0.1
temperature
LB 10C
-1.4
+0.0
-0.2
-0.5
+0.1
motility
outer cut, LB soft agar motility assay
-1.3
-0.2
-0.2
+0.2
-0.3
carbon source
Carbon source D-Mannitol 2.5 mM
-0.1
-0.2
-0.4
-0.9
-0.2
carbon source
L-Lysine (C)
-1.4
-0.2
+0.2
-0.2
-0.1
temperature
LB 10C
-0.3
-0.0
-0.6
-0.8
+0.0
survival
4C survival; 360 hrs
-1.4
+0.1
-0.1
-0.2
+0.1
pH
Minimal media pH8
-0.9
-0.0
-0.2
-0.5
+0.1
stress
methylglyoxal 0.016 vol%
-1.2
-0.3
+0.2
-0.3
+0.1
carbon source
Carbon source D-Glucose 10 mM
-1.0
-0.3
-0.4
+0.3
-0.1
lb
LB
-1.2
+0.1
-0.1
-0.2
+0.0
stress
sodium fluoride 6.25 mM
-0.6
+0.2
-0.6
-0.4
+0.1
stress
Chlorite 0.08 mM
-1.2
+0.1
+0.4
-0.7
+0.1
stress
KB-half, Pseudo11 supernatant, 100 uM FeCl3
+0.1
+0.3
-0.3
-1.1
+0.0
anaerobic
Nitrate reduction with yeast extract
-1.4
-0.1
-0.1
+0.8
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
-0.7
-0.2
+0.2
-0.5
+0.1
carbon source
Carbon source Sucrose 5 mM
+0.4
-0.1
-0.3
-0.9
-0.1
stress
Cobalt chloride 0.08 mM
-0.6
+0.3
+0.1
-0.8
+0.1
carbon source
Carbon source Sucrose 5 mM
-0.9
+0.1
+0.2
-0.2
+0.0
carbon source
D-Gluconic Acid (C)
-0.3
+0.2
+0.2
-0.9
+0.1
carbon source
L-Arabinose (C)
-0.8
-0.0
+0.3
-0.4
+0.1
stress
Spectinomycin 0.025 mg/ml
-0.8
+0.0
+0.5
-0.3
-0.1
carbon source
D-Glucose (C)
-0.3
+0.1
+0.4
-0.8
+0.1
stress
Benzalkonium Chloride 0.016 mg/ml
-0.8
+0.2
+0.6
-0.3
+0.0
stress
Aluminum chloride 5 mM
-0.9
+0.0
+0.5
-0.1
+0.2
motility
outer cut, LB soft agar motility assay
+0.9
-0.1
-0.3
-0.3
-0.1
carbon source
L-Histidine (C)
+0.8
+0.0
-0.4
-0.3
+0.1
stress
Rifampicin 0.0016 mg/ml
+0.5
+0.1
+0.2
-0.6
+0.1
stress
Sisomicin 0.001 mg/ml
+1.0
-0.3
-0.3
+0.3
+0.1
nitrogen source
Urea (N)
+1.0
+0.0
+0.4
-0.4
+0.0
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