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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22480 and AO356_22485 are separated by 63 nucleotides
AO356_22485 and AO356_22490 are separated by 86 nucleotides
AO356_22490 and AO356_22495 are separated by 181 nucleotides
AO356_22495 and AO356_22500 overlap by 4 nucleotides
AO356_22480: AO356_22480 - oxidoreductase, at 5,023,651 to 5,025,216
_22480
AO356_22485: AO356_22485 - hypothetical protein, at 5,025,280 to 5,026,053
_22485
AO356_22490: AO356_22490 - phosphohydrolase, at 5,026,140 to 5,027,579
_22490
AO356_22495: AO356_22495 - RNA polymerase subunit sigma-24, at 5,027,761 to 5,028,267
_22495
AO356_22500: AO356_22500 - hypothetical protein, at 5,028,264 to 5,029,190
_22500
Group
Condition
AO356
_22480
AO356
_22485
AO356
_22490
AO356
_22495
AO356
_22500
carbon source
L-Arginine (C)
-0.1
+0.1
-0.3
-0.2
-0.5
stress
acetate 40 mM
+0.0
-0.3
-0.1
-0.2
-0.3
stress
Ceftazidime 0.2 mg/ml
-0.4
-0.3
-0.2
-0.1
+0.1
carbon source
acetate (C)
-0.3
+0.2
-0.4
-0.1
-0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.0
-0.5
-0.0
+0.2
-0.4
stress
1-ethyl-3-methylimidazolium acetate 10 mM
-0.0
+0.1
-0.3
-0.2
-0.3
stress
Paraquat dichloride 0.0625 mg/ml
-0.3
-0.2
+0.1
-0.2
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
+0.2
-0.2
-0.2
-0.2
nitrogen source
Ammonium chloride (N)
+0.1
+0.1
-0.0
-0.4
-0.4
carbon source
D,L-Lactate (C)
+0.0
+0.2
-0.2
-0.2
-0.3
nitrogen source
Adenine (N)
-0.2
-0.6
+0.2
+0.0
+0.0
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+0.2
-0.2
-0.2
+0.1
-0.3
carbon source
Carbon source D-Mannitol 10 mM
-0.1
-0.2
+0.2
+0.3
-0.5
stress
1-ethyl-3-methylimidazolium lysine 10 mM
-0.3
+0.1
+0.1
-0.2
-0.2
pH
Minimal media pH9
+0.0
-0.4
-0.2
+0.0
+0.3
stress
nitrate 200 mM
+0.1
+0.6
+0.1
-0.5
-0.5
stress
copper (II) chloride 2.5 mM
+0.2
+0.2
+0.4
-0.5
-0.5
motility
outer cut, LB soft agar motility assay
-0.2
-0.3
+0.0
+0.3
-0.1
survival
4C survival; 192 hrs
-0.2
-0.1
-0.1
-0.1
+0.3
carbon source
Carbon source D-Mannitol 5 mM
-0.0
-0.5
+0.3
+0.2
-0.2
anaerobic
Nitrate reduction with yeast extract
+0.3
-0.5
+0.1
+0.2
-0.0
carbon source
Carbon source D-Mannitol 2.5 mM
-0.2
-0.3
+0.2
+0.4
+0.1
stress
Cobalt chloride 0.08 mM
-0.1
+0.6
+0.0
-0.1
-0.2
phage
P5ss-5 phage 10-1 dilution SM calcl2
-0.0
-0.3
-0.2
+0.4
+0.4
stress
Chlorite 0.08 mM
-0.3
+0.2
-0.1
+0.1
+0.4
carbon source
D-Gluconic Acid (C)
+0.0
+0.4
-0.3
+0.1
+0.1
carbon source
D-Lactate (C)
-0.0
+0.6
-0.2
+0.1
-0.1
survival
4C survival; 1152 hrs
+0.2
+0.2
-0.1
+0.0
+0.5
motility
outer cut, LB soft agar motility assay
-0.1
+0.6
+0.6
+0.1
+0.1
stress
KB-half, Pseudo5 supernatant
+0.2
-0.2
-0.3
+0.9
+0.8
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