Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_22315 and AO356_22320 are separated by 241 nucleotidesAO356_22320 and AO356_22325 are separated by 267 nucleotidesAO356_22325 and AO356_22330 overlap by 17 nucleotidesAO356_22330 and AO356_22335 are separated by 7 nucleotides AO356_22315: AO356_22315 - lactate permease, at 4,990,188 to 4,991,951 _22315 AO356_22320: AO356_22320 - thioesterase, at 4,992,193 to 4,992,744 _22320 AO356_22325: AO356_22325 - reactive intermediate/imine deaminase, at 4,993,012 to 4,993,392 _22325 AO356_22330: AO356_22330 - hypothetical protein, at 4,993,376 to 4,993,612 _22330 AO356_22335: AO356_22335 - LysR family transcriptional regulator, at 4,993,620 to 4,994,573 _22335
Group Condition AO356_22315 AO356_22320 AO356_22325 AO356_22330 AO356_22335
nitrogen source Urea (N) +0.1 -0.3 -0.5 -0.8 -0.2
stress 1-ethyl-3-methylimidazolium chloride 200 mM +0.0 -0.2 -0.6 -1.2 +0.3
stress Chlorite 0.04 mM +0.2 -0.1 -0.3 -0.9 -0.0
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.3 +0.0 -0.7 -0.4 -0.2
stress 1-ethyl-3-methylimidazolium acetate 20 mM -0.1 -0.3 -0.4 -0.6 +0.3
carbon source L-Lactate (C) -0.1 -0.3 +0.2 -1.0 +0.2
carbon source L-Lysine (C) +0.2 +0.4 -0.3 -0.9 -0.3
stress Cholin acetate 40 mM +0.1 -0.6 -0.1 -0.5 +0.2
phage P5ss-5 phage 10-1 dilution SM calcl2 +0.5 -1.0 +0.2 -0.0 -0.4
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.3 -0.0 -0.6 -0.4 +0.1
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.4 +0.3 -0.7 -0.6 -0.1
stress Cobalt chloride 0.08 mM -0.2 +0.0 +0.2 -0.9 +0.3
anaerobic Nitrate reduction with yeast extract +0.2 +0.2 +0.4 -1.1 -0.2
stress copper (II) chloride 2.5 mM +0.4 +0.1 +0.6 -1.5 +0.0
carbon source D-Galactose (C) -0.2 +0.1 +0.6 -0.7 -0.0
carbon source D-Gluconic Acid (C) +0.2 +0.1 +0.5 -0.9 -0.0
motility outer cut, LB soft agar motility assay +0.3 +0.3 +0.1 -0.7 -0.1
stress sodium fluoride 6.25 mM -0.0 +0.2 +0.6 -0.8 +0.0
carbon source acetate (C) +0.0 -0.0 +0.7 -0.7 +0.2
carbon source D-Glucose (C) +0.0 -0.5 +0.7 -0.1 +0.1
carbon source L-Lactate (C) +0.1 -0.0 +0.8 -0.8 +0.1
carbon source pyruvate (C) +0.0 -0.2 +0.5 -0.4 +0.2
nitrogen source Putrescine (N) -0.1 +0.3 -0.7 +0.6 +0.0
stress Benzalkonium Chloride 0.008 mg/ml +0.3 -0.4 +0.0 +0.3 +0.3
carbon source Ethanol (C) +0.3 +0.4 -0.2 +0.2 -0.2
stress Lomefloxacin 0.0005 mM -0.4 +0.1 +0.5 +0.5 +0.2
motility outer cut, LB soft agar motility assay -0.3 +0.2 +0.3 +0.4 +0.3
survival 4C survival; 360 hrs -0.1 +0.5 +0.2 +0.0 +0.5
stress KB-half, Pseudo5 supernatant -0.4 +0.6 -0.3 +1.1 +0.2
stress Thallium(I) acetate 0.008 mg/ml +0.5 -0.2 +0.6 +0.2 +0.2
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