Fitness for 5 genes in Pseudomonas fluorescens FW300-N2C3

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500 ntAO356_22230 and AO356_22235 are separated by 267 nucleotidesAO356_22235 and AO356_22240 are separated by 2 nucleotidesAO356_22240 and AO356_22245 are separated by 139 nucleotidesAO356_22245 and AO356_22250 are separated by 129 nucleotides AO356_22230: AO356_22230 - sulfurtransferase, at 4,974,231 to 4,974,623 _22230 AO356_22235: AO356_22235 - DNA repair protein, at 4,974,891 to 4,975,226 _22235 AO356_22240: AO356_22240 - hypothetical protein, at 4,975,229 to 4,975,489 _22240 AO356_22245: AO356_22245 - hypothetical protein, at 4,975,629 to 4,975,979 _22245 AO356_22250: AO356_22250 - GCN5 family acetyltransferase, at 4,976,109 to 4,976,537 _22250
Group Condition AO356_22230 AO356_22235 AO356_22240 AO356_22245 AO356_22250
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. -0.4 -0.2 +0.4 -2.6
motility outer cut, LB soft agar motility assay N.D. -0.8 -0.2 -0.1 -0.8
phage P5ss-5 phage 10-1 dilution SM calcl2 N.D. -0.4 -0.1 -0.2 -1.0
nitrogen source D-Serine (N) N.D. -0.0 -0.5 -0.4 -0.7
stress copper (II) chloride 2.5 mM N.D. -0.6 -0.5 -0.1 -0.2
stress Cholin acetate 40 mM N.D. -0.7 +0.2 -0.2 -0.5
carbon source Cytidine (C) N.D. -0.3 -0.2 -0.3 -0.4
carbon source D-Galactose (C) N.D. -0.3 -0.2 -0.3 -0.3
stress Sisomicin 0.001 mg/ml N.D. -0.8 +0.4 -0.4 -0.2
stress Aluminum chloride 5 mM N.D. +0.1 +0.0 -1.0 +0.0
motility outer cut, LB soft agar motility assay N.D. -0.3 -0.8 +0.4 -0.1
stress Cobalt chloride 0.08 mM N.D. -0.5 +0.0 +0.1 -0.5
stress Cobalt chloride 0.08 mM N.D. -0.4 +0.3 -0.3 -0.4
stress 1-ethyl-3-methylimidazolium lysine 10 mM N.D. -0.3 -0.4 +0.2 -0.2
carbon source D-Ribose (C) N.D. -0.4 +0.2 -0.5 +0.0
carbon source Carbon source D-Mannitol 10 mM N.D. -0.4 -0.1 -0.4 +0.2
carbon source D-Gluconic Acid (C) N.D. -0.4 +0.2 -0.5 +0.0
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. +0.1 +0.2 +0.6 -0.9
stress sodium fluoride 6.25 mM N.D. +0.3 +0.3 -0.3 -0.3
anaerobic Nitrate reduction with yeast extract N.D. -0.2 +0.3 -0.3 +0.3
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. +0.1 +0.4 +0.3 -0.5
motility outer cut, LB soft agar motility assay N.D. -0.4 +0.0 +0.6 +0.1
stress KB-half, Pseudo11 supernatant, 100 uM FeCl3 N.D. +0.3 -0.7 +0.4 +0.3
stress 1-ethyl-3-methylimidazolium acetate 20 mM N.D. -0.2 -0.2 +0.3 +0.4
survival 4C survival; 192 hrs N.D. +0.3 +0.3 -0.3 +0.1
stress Benzethonium chloride 0.025 mM N.D. +0.2 +0.5 +0.1 -0.3
stress KB-half, Pseudo5 supernatant N.D. +0.2 -0.4 +0.5 +0.6
stress nitrate 200 mM N.D. -0.1 +0.5 +0.3 +0.4
stress D-Cycloserine 0.1 mg/ml N.D. +0.1 +0.4 +0.4 +0.2
stress nitrite 10 mM N.D. +0.1 +1.0 -0.0 +0.3
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