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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2C3
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500 nt
AO356_22110 and AO356_22115 are separated by 59 nucleotides
AO356_22115 and AO356_22120 are separated by 176 nucleotides
AO356_22120 and AO356_22125 are separated by 471 nucleotides
AO356_22125 and AO356_22130 are separated by 295 nucleotides
AO356_22110: AO356_22110 - tRNA 2-thiouridylase, at 4,949,408 to 4,950,532
_22110
AO356_22115: AO356_22115 - NUDIX hydrolase, at 4,950,592 to 4,951,038
_22115
AO356_22120: AO356_22120 - hypothetical protein, at 4,951,215 to 4,952,096
_22120
AO356_22125: AO356_22125 - isocitrate dehydrogenase, at 4,952,568 to 4,954,793
_22125
AO356_22130: AO356_22130 - hypothetical protein, at 4,955,089 to 4,955,277
_22130
Group
Condition
AO356
_22110
AO356
_22115
AO356
_22120
AO356
_22125
AO356
_22130
stress
Cholin acetate 40 mM
N.D.
-0.2
-0.3
-2.2
+0.0
stress
acetate 40 mM
N.D.
+0.0
-0.1
-2.2
-0.3
stress
D-Cycloserine 0.1 mg/ml
N.D.
+0.1
-0.1
-2.4
-0.1
stress
1-ethyl-3-methylimidazolium acetate 20 mM
N.D.
+0.1
-0.0
-1.9
-0.5
motility
outer cut, LB soft agar motility assay
N.D.
-0.1
-0.1
-1.3
-0.9
stress
Sisomicin 0.001 mg/ml
N.D.
+0.0
+0.0
-2.1
-0.2
stress
Chlorite 0.04 mM
N.D.
-0.2
-0.1
-1.9
+0.0
stress
1-ethyl-3-methylimidazolium acetate 5 mM
N.D.
+0.1
-0.1
-2.0
-0.1
stress
Cephalothin 2 mg/ml
N.D.
+0.2
-0.0
-1.9
-0.4
stress
Cholin acetate 20 mM
N.D.
+0.2
-0.2
-2.0
-0.2
stress
1-ethyl-3-methylimidazolium acetate 10 mM
N.D.
+0.1
+0.0
-2.2
+0.1
carbon source
Carbon source Sucrose 5 mM
N.D.
-0.0
-0.2
-1.0
-0.6
stress
Bacitracin 2 mg/ml
N.D.
-0.0
+0.0
-1.6
-0.2
stress
Chlorite 0.08 mM
N.D.
+0.1
+0.0
-1.8
-0.0
motility
outer cut, LB soft agar motility assay
N.D.
-0.0
-0.6
-0.7
-0.3
carbon source
Carbon source D-Mannitol 10 mM
N.D.
-0.0
-0.1
-1.4
-0.1
carbon source
Carbon source D-Mannitol 2.5 mM
N.D.
-0.0
-0.0
-1.2
-0.4
carbon source
Carbon source Sucrose 2.5 mM
N.D.
+0.1
-0.0
-1.4
-0.1
stress
syringaldehyde 5 mM
N.D.
+0.1
-0.2
-1.3
-0.1
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
+0.4
-0.2
-1.6
+0.0
carbon source
Carbon source D-Mannitol 5 mM
N.D.
-0.2
-0.1
-1.3
+0.3
stress
nitrite 10 mM
N.D.
-0.3
+0.5
-1.5
+0.2
stress
nitrate 200 mM
N.D.
+0.3
-0.1
-1.4
+0.2
carbon source
L-Lysine (C)
N.D.
+0.3
-0.1
-1.3
+0.1
carbon source
Carbon source D-Mannitol 10 mM
N.D.
+0.2
-0.0
-1.2
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
-0.1
+0.3
-1.3
+0.3
stress
Cisplatin 0.0032 mg/ml
N.D.
+0.2
+0.3
-0.5
-0.7
resistance
50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
N.D.
+0.0
-0.0
+0.5
-1.1
carbon source
acetate (C)
N.D.
+0.2
+0.1
+2.0
-0.1
carbon source
acetate (C)
N.D.
+0.3
+0.0
+1.7
+0.1
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